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MOUSE Anti-HISTIDINE TAG:FITC Antibody

MOUSE Anti-HISTIDINE TAG:FITC Antibody size: 100ug 464

Supplier MBS MONO
Price 464
Size 100ug
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Also known asHISTIDINE TAG
Other namesN/A
CategoryAntibodies
SubcategoryMnoclonal antibodies
Gene nameN/A
Gene name synonimsN/A
Other gene namesN/A
ClonalityMonoclonal
Immunoglobulin isotypeIgG1
Clone1, 10, AD1
Host organismMouse (Mus musculus)
Species reactivity Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Synthetic Peptide
Specificity and cross-reactivity In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, It reacts against all His tagged proteins so far tested, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, The antibody may be used to track the purification of proteins expressed in bacteria, This particular antibody might cross react with speacies outside of the listed ones, although some cross-reactivity may be seen with extracts of insect or mammalian cells, yeast or insect cells, HISTIDINE TAG This item recognises proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags
Purification methodN/A
Form/AppearanceFITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)
Concentration1 miligram per 1 mililiter, IgG concentration 0
Storage and shipping If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use, Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided, Shelf Life: 18 months from date of dispatch, Storage in frost free freezers is not recommended, the antibody is photosensitive and should be protected from light, Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term, the antibody should be stored undiluted
Tested applications: Immunofluorescence (IF), Flow Cytometry (FACS)
Properties FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, MBS mono FITC is highly sensitive to pH extremes, This MBS mono Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24°
ConjugationAnti-FITC Antibody
Gene targetHISTIDINE TAG:
Short nameMOUSE Anti-HISTIDINE TAG:FITC Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, Antibody
LabelFITC
Alternative nameMOUSE antibody to-HISTIDINE detection labelled:fluorecein (Antibody to)
Alternative techniqueantibodies

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