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MOUSE Anti-HUMAN CD33:FITC Antibody

MOUSE Anti-HUMAN CD33:FITC Antibody size: 100ug 448

Supplier MBS MONO
Price 448
Size 100ug
Also known asCD33
Other names CD33 antigen (gp67), CD33 molecule, Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67, gp67, myeloid cell surface antigen CD33, sialic acid binding Ig-like lectin 3, sialic acid-binding Ig-like lectin 3, myeloid cell surface antigen CD33 isoform 2
CategoryAntibodies
SubcategoryMnoclonal antibodies
Gene nameCD33
Gene name synonimsN/A
Other gene names CD33, SIGLEC-3, SIGLEC3, SIGLEC3, Siglec-3, p67, CD33
ClonalityMonoclonal
Immunoglobulin isotypeIgG1
CloneWM53
Host organismMouse (Mus musculus)
Species reactivity Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Human (Homo sapiens)
Specificity and cross-reactivity For lymphoid cells, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, This antigen, This particular antibody might cross react with speacies outside of the listed ones, Using clone WM53, a smaller protein of approximately 67kDa has been observed, considered to be specific for the myeloid lineage, has also been reported to be present on cells of lymphoid origin, the CD33 protein immunoprecipitated from myeloid cells is approximately 75kDa in size, CD33 This item recognises the human CD33 cell surface glycoprotein
Purification methodN/A
Form/AppearanceFITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)
Concentration1 miligram per 1 mililiter, IgG concentration 0
Storage and shipping If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use, Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided, Shelf Life: 18 months from date of dispatch, Storage in frost free freezers is not recommended, the antibody is photosensitive and should be protected from light, the antibody should be stored undiluted, Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term
Tested applications:Flow Cytometry (FACS)
Properties FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, MBS mono FITC is highly sensitive to pH extremes, This MBS mono Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24°
ConjugationAnti-FITC Antibody
Gene targetCD33:
Short nameMOUSE Anti-HUMAN CD33:FITC Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, Antibody
LabelFITC
Alternative name sapiens CD33 molecule:fluorecein (Antibody to), MOUSE antibody to-H
Alternative techniqueantibodies
Alternative to gene target CD33 and IDBG-65737 and ENSG00000105383 and 945, Cell surfaces, p67 and SIGLEC-3 and SIGLEC3, protein binding, this GO :0004872 and receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0007155 and cell adhesion and biological process this GO :0007165 and signal transduction and biological process this GO :0007267 and cell-cell signaling and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0009897 and external side of plasma membrane and cellular component this GO :0070062 and extracellular vesicular exosome and cellular component, this GO :0004872: receptor activity, this GO :0004872: receptor activity and also this GO :0005515: protein binding, this GO :0005515: protein binding, CD33 molecule
Identity 1659
Gene CD33
Long gene name CD33 molecule
Synonyms gene name CD33 antigen (gp67)
Synonyms SIGLEC3 SIGLEC-3 p67 FLJ00391
Synonyms name sialic acid binding Ig-like lectin 3
Locus 19q13, 41
Discovery year 1986-01-01
GenBank acession M23197
Entrez gene record 945
Pubmed identfication 3139766 9465907
RefSeq identity NM_001772
Classification CD molecules Sialic acid binding Ig like lectins V-set domain containing
Havana BLAST/BLAT OTTHUMG00000182891

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