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MOUSE Anti-HUMAN CD50:FITC Antibody

MOUSE Anti-HUMAN CD50:FITC Antibody size: 100ug 448

Supplier MBS MONO
Price 448
Size 100ug
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Also known asCD50
Other names CDw50, ICAM-3, ICAM-R, Intercellular adhesion molecule 3, intercellular adhesion molecule 3, intercellular adhesion molecule 3, intercellular adhesion molecule-3, intercellular adhesion molecule 3
CategoryAntibodies
SubcategoryMnoclonal antibodies
Gene nameCD50
Gene name synonimsN/A
Other gene names CD50, CDW50, ICAM-3, ICAM-R, ICAM3, ICAM3
ClonalityMonoclonal
Immunoglobulin isotypeIgG1
CloneMEM-171
Host organismMouse (Mus musculus)
Species reactivity Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Human (Homo sapiens)
Specificity and cross-reactivity CD50 is a type I transmembrane glycoprotein, CD50 is expressed by a wide range of leucocytes, Clone MEM-171 recognises an epitope in the D2 domain of human CD50, It plays a role in leucocyte adhesion and also acts as a signaling and costimulatory molecule on T lymphocytes, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, This clone is reported to partially inhibit CD43 induced apoptosis of TF-1 cells, This particular antibody might cross react with speacies outside of the listed ones, a 110kD molecule also known as ICAM-3, and is a ligand for LFA-1, containing five immunoglobulin-like C2-type domains, CD50 This item recognises the human CD50 cell surface glycoprotein
Purification methodN/A
Form/AppearanceFITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)
Concentration1 miligram per 1 mililiter, IgG concentration 0
Storage and shipping If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use, Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided, Shelf Life: 18 months from date of dispatch, Storage in frost-free freezers is not recommended, the antibody is photosensitive and should be protected from light, the antibody should be stored undiluted, Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term
Tested applications: Immunoprecipitation (IP), Flow Cytometry (FACS)
Properties FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, MBS mono FITC is highly sensitive to pH extremes, This MBS mono Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24°
ConjugationAnti-FITC Antibody
Gene targetCD50:
Short nameMOUSE Anti-HUMAN CD50:FITC Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, Antibody
LabelFITC
Alternative name sapiens CD50:fluorecein (Antibody to), MOUSE antibody to-H
Alternative techniqueantibodies
Identity 5346
Gene ICAM3
Long gene name intercellular adhesion molecule 3
Synonyms CDW50 ICAM-R CD50
Locus 19p13, 2
Discovery year 1994-05-19
Entrez gene record 3385
Pubmed identfication 1448174
Classification CD molecules Immunoglobulin like domain containing
Havana BLAST/BLAT OTTHUMG00000180410

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