Name : MOUSE Anti-PIG CD4a:FITC Antibody
Supplier : MBS MONO
Price :713
SKU : GEN3845912658
| Also known as | CD4a |
| Other names | N/A |
| Category | Antibodies |
| Subcategory | Mnoclonal antibodies |
| Gene name | CD4a |
| Gene name synonims | N/A |
| Other gene names | N/A |
| Clonality | Monoclonal |
| Immunoglobulin isotype | IgG2b |
| Clone | MIL17 |
| Host organism | Mouse (Mus musculus) |
| Species reactivity | Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Pig |
| Specificity and cross-reactivity | In addition to the two populations of mutually exclusive CD4+/CD8- and CD4-/CD8+ lymphocytes, Lymphoblasts with a double positive phenotype have been described in other species but this is not the case for mature T lymphocytic calls _x000D_MIL17 stains a population of cells with characteristic lymphocyte morphology in immunohistochemistry, MIL-17 was confirmed as a member of the CD4a cluster at the 'Third International Workshop on Swine Leukocyte Differentiation Antigens' Porcine CD4 is a type 1 trans-membrane member of the immunoglobulin superfamily, Pigs appear unusual amongst mammalian species as they appear to have four populations of resting T lymphocytes, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, This particular antibody might cross react with speacies outside of the listed ones, clone MIL17 recognises a 55kDa porcine homologue to the human CD4 antigen found on the surface of helper-T cells, they also appear to have significant populations of CD4-/CD8- and CD4+/CD8+ cells, CD4a Mouse anti pig CD4a |
| Purification method | N/A |
| Form/Appearance | FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid) |
| Concentration | 1 miligram per 1 mililiter, IgG concentration 0 |
| Storage and shipping | If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use, Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided, Shelf Life: 18 months from date of dispatch, Storage in frost free freezers is not recommended, the antibody is photosensitive and should be protected from light, the antibody should be stored undiluted, Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term |
| Tested applications: | Flow Cytometry (FACS) |
| Properties | FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, MBS mono FITC is highly sensitive to pH extremes, This MBS mono Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24° |
| Conjugation | Anti-FITC Antibody |
| Gene target | CD4a: |
| Short name | MOUSE Anti-PIG CD4a:FITC Antibody |
| Technique | Fluorescein, antibodies against human proteins, antibodies for, Antibody |
| Label | FITC |
| Alternative name | MOUSE antibody to-swine CD4a:fluorecein (Antibody to) |
| Alternative technique | antibodies |