Name : MOUSE Anti-RAT CD45:FITC Antibody
Supplier : MBS MONO
Price :277
SKU : GEN3580425574
| Also known as | CD45 |
| Other names | C, L-CA, Leukocyte common antigen, Protein tyrosine phosphatase, Receptor-type tyrosine-protein phosphatase C, T200, T200 glycoprotein, c polypeptide, leucocyte common antigen, leukocyte common antigen A, leukocyte common antigen B, protein tyrosine phosphatase, receptor type, receptor-type, receptor-type tyrosine-protein phosphatase C, receptor-type tyrosine-protein phosphatase C isoform 3 |
| Category | Antibodies |
| Subcategory | Mnoclonal antibodies |
| Gene name | CD45 |
| Gene name synonims | N/A |
| Other gene names | CD45, L-CA, L-CA, Lca, Ptprc, RT7, T200, Ptprc |
| Clonality | Monoclonal |
| Immunoglobulin isotype | IgG1 |
| Clone | OX-1 |
| Host organism | Mouse (Mus musculus) |
| Species reactivity | Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Rat (Rattus norvegicus) |
| Specificity and cross-reactivity | CD45RO etc, OX-1 reacts with all forms of CD45 expressed by all haematopoietic cells, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, The leucocyte common antigen consists of a family of heavily glycosylated membrane glycoproteins of molecular weight 180 - 240kDa, This particular antibody might cross react with speacies outside of the listed ones, _x000D_ _x000D_This product is routinely tested by flow cytometry on rat splenocytes, _x000D__x000D_Antibodies recognising a common epitope on all of these isoforms are termed CD45, _x000D__x000D_CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction, also known as the leucocyte common antigen (LCA), except erythrocytes, whilst those recognising only individual isoforms are termed CD45RA, CD45 This item recognises CD45 |
| Purification method | N/A |
| Form/Appearance | FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid) |
| Concentration | 1 miligram per 1 mililiter, IgG concentration 0 |
| Storage and shipping | If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use, Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided, Shelf Life: 18 months from date of dispatch, Storage in frost free freezers is not recommended, the antibody is photosensitive and should be protected from light, the antibody should be stored undiluted, Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term |
| Tested applications: | Flow Cytometry (FACS) |
| Properties | FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, MBS mono FITC is highly sensitive to pH extremes, This MBS mono Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24° |
| Conjugation | Anti-FITC Antibody |
| Gene target | CD45: |
| Short name | MOUSE Anti-RAT CD45:FITC Antibody |
| Technique | Fluorescein, antibodies against human proteins, antibodies for, Antibody |
| Label | FITC |
| Alternative name | MOUSE antibody to-RAT CD45:fluorecein (Antibody to) |
| Alternative technique | antibodies |
| Identity | 9666 |
| Gene | PTPRC |
| Long gene name | protein tyrosine phosphatase, receptor type C |
| Synonyms gene | CD45 |
| Synonyms | LCA T200 GP180 |
| Locus | 1q31, 1 , 3-q32 |
| Discovery year | 1986-01-01 |
| GenBank acession | Y00062 |
| Entrez gene record | 5788 |
| Pubmed identfication | 2169617 |
| RefSeq identity | NM_002838 |
| Classification | Fibronectin type III domain containing CD molecules Protein tyrosine phosphatases, receptor type |
| Havana BLAST/BLAT | OTTHUMG00000035702 |
| Locus Specific Databases | PTPRCbase: Mutation registry for CD45 deficiency LRG_95 |