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MOUSE Anti-RAT MHC CLASS II RT1B:Biotin Antibody

MOUSE Anti-RAT MHC CLASS II RT1B:Biotin Antibody size: 100 Tests 359

Supplier MBS MONO
Price 359
Size 100 Tests
Also known asMHC CLASS II RT1B
Other namesN/A
CategoryAntibodies
SubcategoryMnoclonal antibodies
Gene nameMHC CLASS II RT1B
Gene name synonimsN/A
Other gene namesN/A
ClonalityMonoclonal
Immunoglobulin isotypeIgG1
CloneOX-6
Host organismMouse (Mus musculus)
Species reactivity Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Rat (Rattus norvegicus)
Specificity and cross-reactivity Analysis of recombinant mouse strains has mapped the OX-6 determinant to the H-2I-A region This product is routinely tested in flow cytometry on rat splenocytes, In mice, In rats, MBS213028 also cross reacts with a polymorphic determinant on mouse strains of the H-2 haplotypes k and s, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, The literature reports that this clone does not react with the rat BDIX strain due to a defect in RT1B expression<, This item recognises a monomorphic determinant of the rat RT1B MHC class II antigen present on B lymphocytes, This particular antibody might cross react with speacies outside of the listed ones, dendritic cells, some macrophages and certain epithelial cells, this complex is referred to as the H-2 region, this complex is referred to as the RT1 region, /sup>, MHC CLASS II RT1B The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections
Purification methodN/A
Form/AppearanceBiotin (Purified IgG conjugated to Biotin - lyophilised)
ConcentrationN/A
Storage and shipping Following reconstitution Store the antibody at +4 degrees Celsius, If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use, Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided, Shelf Life: 12 months from date of reconstitution, Temperature variations in the range between +1C to +7C are tolerable, for longer, for one month or the antibody should be stored at -20 degrees Celsius, the antibody should be stored undiluted, Prior to reconstitution Keep the antibody refrigerated at +4 degrees Celsius
Tested applications:Flow Cytometry (FACS)
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24°
ConjugationBiotinylated
Gene targetMHC CLASS II RT1B:
Short nameMOUSE Anti-RAT MHC CLASS II RT1B: Antibody
Technique antibodies against human proteins, antibodies for, Antibody
LabelBiotin
Alternative nameMOUSE antibody to-RAT Major histocompabitility complex CLASS II RT1B:biotinilated (Antibody to)
Alternative techniqueantibodies

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