Name : MOUSE Anti-RAT MHC CLASS II RT1B:Biotin Antibody
Supplier : MBS MONO
Price :359
SKU : GEN1469371269
| Also known as | MHC CLASS II RT1B |
| Other names | N/A |
| Category | Antibodies |
| Subcategory | Mnoclonal antibodies |
| Gene name | MHC CLASS II RT1B |
| Gene name synonims | N/A |
| Other gene names | N/A |
| Clonality | Monoclonal |
| Immunoglobulin isotype | IgG1 |
| Clone | OX-6 |
| Host organism | Mouse (Mus musculus) |
| Species reactivity | Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Rat (Rattus norvegicus) |
| Specificity and cross-reactivity | Analysis of recombinant mouse strains has mapped the OX-6 determinant to the H-2I-A region This product is routinely tested in flow cytometry on rat splenocytes, In mice, In rats, MBS213028 also cross reacts with a polymorphic determinant on mouse strains of the H-2 haplotypes k and s, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, The literature reports that this clone does not react with the rat BDIX strain due to a defect in RT1B expression<, This item recognises a monomorphic determinant of the rat RT1B MHC class II antigen present on B lymphocytes, This particular antibody might cross react with speacies outside of the listed ones, dendritic cells, some macrophages and certain epithelial cells, this complex is referred to as the H-2 region, this complex is referred to as the RT1 region, /sup>, MHC CLASS II RT1B The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections |
| Purification method | N/A |
| Form/Appearance | Biotin (Purified IgG conjugated to Biotin - lyophilised) |
| Concentration | N/A |
| Storage and shipping | Following reconstitution Store the antibody at +4 degrees Celsius, If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use, Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided, Shelf Life: 12 months from date of reconstitution, Temperature variations in the range between +1C to +7C are tolerable, for longer, for one month or the antibody should be stored at -20 degrees Celsius, the antibody should be stored undiluted, Prior to reconstitution Keep the antibody refrigerated at +4 degrees Celsius |
| Tested applications: | Flow Cytometry (FACS) |
| Properties | Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24° |
| Conjugation | Biotinylated |
| Gene target | MHC CLASS II RT1B: |
| Short name | MOUSE Anti-RAT MHC CLASS II RT1B: Antibody |
| Technique | antibodies against human proteins, antibodies for, Antibody |
| Label | Biotin |
| Alternative name | MOUSE antibody to-RAT Major histocompabitility complex CLASS II RT1B:biotinilated (Antibody to) |
| Alternative technique | antibodies |