Name : MOUSE Anti-RAT MHC CLASS II RT1Bu/l:Biotin Antibody
Supplier : MBS MONO
Price :332
SKU : GEN9351645473
| Also known as | MHC CLASS II RT1Bu/l |
| Other names | N/A |
| Category | Antibodies |
| Subcategory | Mnoclonal antibodies |
| Gene name | N/A |
| Gene name synonims | N/A |
| Other gene names | N/A |
| Clonality | Monoclonal |
| Immunoglobulin isotype | IgG1 |
| Clone | OX-3 |
| Host organism | Mouse (Mus musculus) |
| Species reactivity | Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Rat (Rattus norvegicus) |
| Specificity and cross-reactivity | Analysis of recombinant mouse strains has mapped the OX-3 determinant to the H-2I-A region, DA or PVG/c strains (1, In mice, In rats, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, The literature reports reactivity with Lewis, This antibody is useful for distinguishing RT1B positive cells from different rat strains, This item recognises a polymorphic determinant of the rat RT1B MHC class II antigen, This particular antibody might cross react with speacies outside of the listed ones, Wistar and AO strain rats but not BN, _x000D_This item also cross reacts with mouse strains of the H-2 haplotypes b and s, _x000D_This product is routinely tested in flow cytometry on Lewis rat splenocytes, e, for recognising cells of donor origin in bone marrow reconstituted radiation chimaeras (3, reacting with haplotypes u and l, this complex is referred to as the H-2 region, this complex is referred to as the RT1 region, 2), 4), MHC CLASS II RT1Bu/l The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections, g |
| Purification method | N/A |
| Form/Appearance | Biotin (Purified IgG conjugated to Biotin - liquid) |
| Concentration | 1 miligram per 1 mililiter, IgG concentration 0 |
| Storage and shipping | If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use, Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided, Shelf Life: 18 months from date of dispatch, or -20<, the antibody should be stored undiluted Storage in frost free freezers is not recommended, /sup>, C iif preferred, Store the antibody at +4 degrees Celsius |
| Tested applications: | Flow Cytometry (FACS) |
| Properties | Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24° |
| Conjugation | Biotinylated |
| Gene target | MHC CLASS II RT1Bu/l: |
| Short name | MOUSE Anti-RAT MHC CLASS II RT1Bu/l: Antibody |
| Technique | antibodies against human proteins, antibodies for, Antibody |
| Label | Biotin |
| Alternative name | MOUSE antibody to-RAT Major histocompabitility complex CLASS II RT1Bu/l:biotinilated (Antibody to) |
| Alternative technique | antibodies |