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Mouse Monoclonal [clone N-GAD65] (IgG1) to Human GAD65 Antibody

Mouse Monoclonal [clone N-GAD65] (IgG1) to Human GAD65 Antibody size: 50ug 597

Supplier MBS MONO
Price 597
Size 50ug
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Also known asGAD65
Other names 65 kDa glutamic acid decarboxylase, 65 kDa glutamic acid decarboxylase, 65kDa), GAD-65, GAD-65, Glutamate decarboxylase 2, Glutamate decarboxylase 65 kDa isoform, Glutamate decarboxylase-2 (pancreas), glutamate decarboxylase 2, glutamate decarboxylase 2 (pancreatic islets and brain, glutamate decarboxylase 65 kDa isoform, glutamate decarboxylase 2
CategoryAntibodies
SubcategoryMnoclonal antibodies
Gene nameGAD65
Gene name synonimsGAD2
Other gene names GAD-65, GAD2, GAD65, GAD65, GAD2
ClonalityMonoclonal
Immunoglobulin isotypeIgG1
CloneN-GAD65
Host organismMouse (Mus musculus)
Species reactivity Due to limited knowledge and inability for testing each and every species, Monkey, Rat (Rattus norvegicus), the reactivity of the antibody may extend to other species which are not listed hereby, Human (Homo sapiens)
Specificity and cross-reactivity Clone N-GAD65 has been shown to be highly specific for GAD65 and does not recognize GAD67, GAD65 catalyzes the decarboxylation of glutamate to GABA, GAD65 is the 65kD isoform of GAD, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, Studies have shown the presence of autoantibodies to GAD65 (GAD65Ab) in autoimmune diseases, The N-Terminal region of GAD65 lacks the epitopes for GAD65Ab in Type I diabetes patients, This particular antibody might cross react with speacies outside of the listed ones, an amphiphilic membrane-anchored protein and member of the group 2 decarboxylase family, and has been shown as essential for targeting the enzyme to GABA-containing secretory vesicles, and is encoded by the GAD1 gene, and those at high risk of developing Type I diabetes, as oppose to the 67kD isoform (GAD67), but GAD65Ab are most prevalent in patients with Type I diabetes mellitus, encoded by the GAD2 gene, including Graves disease and Stiff Man Syndrome (SMS), otherwise known as GAD65/GAD2, principally expressed in the brain and also in pancreatic beta cells, the major inhibitory neurotransmitter in the central nervous system, which is predominantly expressed by nerve termini, which is predominantly found in the cell body, Specifically recognizes an epitope within the N-terminal (NT) region of Glutamate decarboxylase 2
Purification methodProtein G Purified
Form/Appearance 0, 09% sodium azide, PBS
Concentration1 miligram per 1 mililiter
Storage and shipping Cycles of freezing and defrosting may denaturate the polypeptide chains of the antibodies and reduce their activity, For longer storage the antibody should be kept at -20 degrees Celsius, For short term storage keep the antibody at +4 degrees Celsius
Tested applications: Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blot (WB), Immunohistochemistry (IHC - Paraffin)
Properties Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Human proteins, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine,  , (Homo sapiens, C, C for long term storage and for short term at + 5°, Homo sapiens sapiens), If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24°, humans , ssp
About Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Monoclonals of this antigen are available in different clones
Test A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or 
Latin nameMus musculus
Gene targetMonoclonal [ N-GAD65] (IgG1) GAD65
Short nameMouse Monoclonal [clone N-GAD65] (IgG1) GAD65 Antibody
Technique Monoclonals or monoclonal antibodies, antibodies against human proteins, antibodies for, mouses, Antibody
IsotypeIgG1
Species Humans, Mouses, Human
Alternative name sapiens GAD65 (Antibody to), Mouse monoclonal [clonality N-GAD65] (IgG1) to H
Alternative techniqueantibodies
Identity 4093
Gene GAD2
Long gene name glutamate decarboxylase 2
Synonyms gene name 65kD) glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) , glutamate decarboxylase 2 (pancreatic islets and brain
Synonyms GAD65
Locus 10p12, 1
Discovery year 1991-11-29
GenBank acession AJ251501
Entrez gene record 2572
Pubmed identfication 2039509
RefSeq identity NM_000818
Havana BLAST/BLAT OTTHUMG00000017836

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