| Also known as | ZAP70 |
| Other names | 70 kDa zeta-associated protein, 70 kDa zeta-chain associated protein, 70 kDa zeta-chain associated protein, 70kD, Syk-related tyrosine kinase, Tyrosine-protein kinase ZAP-70, syk-related tyrosine kinase, tyrosine-protein kinase ZAP-70, zeta-chain (TCR) associated protein kinase 70kDa, zeta-chain associated protein kinase, tyrosine-protein kinase ZAP-70 isoform 1 |
| Category | Antibodies |
| Subcategory | Mnoclonal antibodies |
| Gene name | ZAP70 |
| Gene name synonims | N/A |
| Other gene names | SRK, SRK, STCD, STD, TZK, ZAP-70, ZAP70, ZAP70 |
| Clonality | Monoclonal |
| Immunoglobulin isotype | IgG1 |
| Clone | ZAP-03 |
| Host organism | Mouse (Mus musculus) |
| Species reactivity | Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Human (Homo sapiens) |
| Specificity and cross-reactivity | Recent studies suggest that ZAP-70 is also expressed in a population of normal immature B-cells and in B-cells from a sub-set of patients with chronic lymphocytic leukemia, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, This particular antibody might cross react with speacies outside of the listed ones, ZAP-70 is primarily expressed in T lymphocytes and natural killer cells, a 70kD tyrosine protein kinase which associates with the T-cell receptor (TCR) zeta chain and undergoes phosphorylation following TCR stimulation, where it plays a key role in T-cell receptor (TCR) signalling, Recognizes human ZAP-70 |
| Purification method | Protein A Purified |
| Form/Appearance | 0, pH 7, 09% sodium azide, 4, PBS |
| Concentration | 1 miligram per 1 mililiter |
| Storage and shipping | For long term storage aliquot and keep the antibody at -20 degrees Celsius, Freeze-thaw cycles should be avoided in order to retain the activity of the antibody, Store at +4 degrees Celsius for short term storage |
| Tested applications: | Flow Cytometry (FC/FACS), Western Blot (WB), Immunohistochemistry (IHC - Paraffin) |
| Properties | Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Human proteins, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine, , (Homo sapiens, C, C for long term storage and for short term at + 5°, Homo sapiens sapiens), If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24°, humans , ssp |
| About | Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Monoclonals of this antigen are available in different clones |
| Test | A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Latin name | Mus musculus |
| Gene target | Monoclonal [ ZAP-03] (IgG1) ZAP70 |
| Short name | Mouse Monoclonal [clone ZAP-03] (IgG1) ZAP70 Antibody |
| Technique | Monoclonals or monoclonal antibodies, antibodies against human proteins, antibodies for, mouses, Antibody |
| Isotype | IgG1 |
| Species | Humans, Mouses, Human |
| Alternative name | sapiens zeta-chain (TCR) associated protein kinase 70kDa (Antibody to), Mouse monoclonal [clonality ZAP-03] (IgG1) to H |
| Alternative technique | antibodies |
| Alternative to gene target | Cell surfaces, SRK and STCD and STD and TZK and ZAP-70, ZAP70 and IDBG-62509 and ENSG00000115085 and 7535, ZAP70 and IDBG-629842 and ENSBTAG00000005892 and 504509, Zap70 and IDBG-145089 and ENSMUSG00000026117 and 22637, this GO :0001772 and immunological synapse and cellular component this GO :0001784 and phosphotyrosine binding and molecular function this GO :0002250 and adaptive immune response and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0004715 and non-membrane spanning protein tyrosine kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005911 and cell-cell junction and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0006955 and immune response and biological process this GO :0016772 and transferase activity, this GO :0001784: phosphotyrosine binding, this GO :0001784: phosphotyrosine binding and also this GO :0004672: protein kinase activity and also this GO :0004713: protein tyrosine kinase activity and also this GO :0004715: non-membrane spanning protein tyrosine kinase activity and also this GO :0005515: protein binding and also this GO :0005524: ATP binding and also this GO :0016772: transferase activity, this GO :0004672: protein kinase activity, this GO :0004713: protein tyrosine kinase activity, this GO :0004715: non-membrane spanning protein tyrosine kinase activity, this GO :0005515: protein binding, this GO :0005524: ATP binding, this GO :0016772: transferase activity, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups, transferring phosphorus-containing groups and molecular function this GO :0018108 and peptidyl-tyrosine phosphorylation and biological process this GO :0030217 and T cell differentiation and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0042101 and T cell receptor complex and cellular component this GO :0042110 and T cell activation and biological process this GO :0042113 and B cell activation and biological process this GO :0043366 and beta selection and biological process this GO :0045059 and positive thymic T cell selection and biological process this GO :0045060 and negative thymic T cell selection and biological process this GO :0045061 and thymic T cell selection and biological process this GO :0045121 and membrane raft and cellular component this GO :0045582 and positive regulation of T cell differentiation and biological process this GO :0046638 and positive regulation of alpha-beta T cell differentiation and biological process this GO :0046641 and positive regulation of alpha-beta T cell proliferation and biological process this GO :0046777 and protein autophosphorylation and biological process this GO :0050850 and positive regulation of calcium-mediated signaling and biological process this GO :0050852 and T cell receptor signaling pathway and biological process this GO :0070489 and T cell aggregation and biological process this GO :0072678 and T cell migration and biological process, zeta-chain (TCR) associated protein kinase 70kDa |
| Identity | 12858 |
| Gene | ZAP70 |
| Long gene name | zeta chain of T-cell receptor associated protein kinase 70 |
| Synonyms gene | SRK |
| Synonyms gene name | zeta-chain (TCR) associated protein kinase (70 kD) zeta-chain (TCR) associated protein kinase 70kDa zeta chain of T cell receptor associated protein kinase 70kDa zeta chain of T cell receptor associated protein kinase 70 |
| Synonyms | ZAP-70 STD |
| Synonyms name | tyrosine-protein kinase ZAP-70 |
| Locus | 2q11, 2 |
| Discovery year | 1995-05-16 |
| GenBank acession | L05148 |
| Entrez gene record | 7535 |
| Pubmed identfication | 1423621 |
| RefSeq identity | NM_001079 |
| Classification | SH2 domain containing |
| Havana BLAST/BLAT | OTTHUMG00000153060 |
| Locus Specific Databases | ZAP70base: Mutation registry for autosomal recessive ZAP70 immunodeficiency LRG_126 |
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