| Also known as | CFLAR / FLIP |
| Other names | CASH, CASP8 and FADD-like apoptosis regulator, CASP8 and FADD-like apoptosis regulator, CASP8 and FADD-like apoptosis regulator, CASP8 and FADD-like apoptosis regulator subunit p12, CLARP, Caspase homolog, Caspase-eight-related protein, Caspase-like apoptosis regulatory protein, Casper, Cellular FLICE-like inhibitory protein, FADD-like antiapoptotic molecule 1, FADD-like antiapoptotic molecule 1, FLAME-1, I-FLICE, Inhibitor of FLICE, MACH-related inducer of toxicity, MACH-related inducer of toxicity, MRIT, UsurpinCleaved into the following 2 chains:CASP8 and FADD-like apoptosis regulator subunit p43, c-FLIP, caspase homolog, caspase-eight-related protein, caspase-like apoptosis regulatory protein, caspase-related inducer of apoptosis, cellular FLICE-like inhibitory protein, inhibitor of FLICE, usurpin beta, CASP8 and FADD-like apoptosis regulator isoform 1 |
| Category | Antibodies |
| Subcategory | Mnoclonal antibodies |
| Gene name | CFLAR |
| Gene name synonims | FLIP |
| Other gene names | CASH, CASH, CASH, CASP8AP1, CASP8AP1, CFLAR, CLARP, CLARP, CLARP, Casper, Casper, FLAME, FLAME-1, FLAME-1, FLAME1, FLIP, I-FLICE, I-FLICE, MRIT, MRIT, MRIT, c-FLIP, c-FLIP, c-FLIPL, c-FLIPR, c-FLIPS, CFLAR |
| Clonality | Monoclonal |
| Immunoglobulin isotype | IgG2a, k |
| Clone | 2F12 |
| Host organism | Mouse (Mus musculus) |
| Species reactivity | Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Human (Homo sapiens) |
| Specificity and cross-reactivity | Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, This particular antibody might cross react with speacies outside of the listed ones, Human CFLAR |
| Purification method | Protein A Purified |
| Form/Appearance | Sourced in Ascites, pH 7, 2, PBS |
| Concentration | 5 mg/ml |
| Storage and shipping | For long term storage aliquot and keep the antibody at -20 degrees Celsius, Freeze-thaw cycles should be avoided in order to retain the activity of the antibody, Store at +4 degrees Celsius for short term storage |
| Tested applications: | ELISA (EIA), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC - Paraffin) |
| Properties | Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Human proteins, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine, , (Homo sapiens, C, C for long term storage and for short term at + 5°, Homo sapiens sapiens), If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24°, humans , ssp |
| About | Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Monoclonals of this antigen are available in different clones |
| Test | A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Latin name | Mus musculus |
| Gene target | Monoclonal [ 2F12] (IgG2a, k) CFLAR / FLIP |
| Short name | Mouse Monoclonal [clone 2F12] (IgG2a, k) CFLAR / FLIP Antibody |
| Technique | Monoclonals or monoclonal antibodies, antibodies against human proteins, antibodies for, mouses, Antibody |
| Isotype | IgG2a |
| Species | Humans, Mouses, Human |
| Alternative name | member 6) (TNFRSF6)-associated via death domain-like apoptosis regulator / FLIP (Antibody to), sapiens CASP8 and Fas (TNF receptor superfamily, Mouse monoclonal [clonality 2F12] (IgG2a, k) to H |
| Alternative technique | antibodies |
| Alternative to gene target | CFLAR and IDBG-78386 and ENSG00000003402 and 8837, FLIP and IDBG-643202 and ENSBTAG00000010998 and 497199, Plasma membranes, c-FLIP and c-FLIPL and c-FLIPR and c-FLIPS and CASH and CASP8AP1 and Casper and CLARP and FLAME and FLAME-1 and FLAME1 and FLIP and I-FLICE and MRIT, death effector domain binding, this GO :0002020 and protease binding and molecular function this GO :0004197 and cysteine-type endopeptidase activity and molecular function this GO :0005123 and death receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0006508 and proteolysis and biological process this GO :0006915 and apoptotic process and biological process this GO :0007519 and skeletal muscle tissue development and biological process this GO :0008047 and enzyme activator activity and molecular function this GO :0008234 and cysteine-type peptidase activity and molecular function this GO :0014732 and skeletal muscle atrophy and biological process this GO :0014842 and regulation of satellite cell proliferation and biological process this GO :0014866 and skeletal myofibril assembly and biological process this GO :0016032 and viral process and biological process this GO :0031264 and death-inducing signaling complex and cellular component this GO :0031265 and CD95 death-inducing signaling complex and cellular component this GO :0032403 and protein complex binding and molecular function this GO :0035877 and death effector domain binding and molecular function this GO :0042981 and regulation of apoptotic process and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043085 and positive regulation of catalytic activity and biological process this GO :0043123 and positive regulation of I-kappaB kinase/NF-kappaB signaling and biological process this GO :0043403 and skeletal muscle tissue regeneration and biological process this GO :0045087 and innate immune response and biological process this GO :0045121 and membrane raft and cellular component this GO :0051092 and positive regulation of NF-kappaB transcription factor activity and biological process this GO :0060544 and regulation of necroptotic process and biological process this GO :0097190 and apoptotic signaling pathway and biological process this GO :0097342 and ripoptosome and cellular component this GO :1901740 and negative regulation of myoblast fusion and biological process this GO :1902042 and negative regulation of extrinsic apoptotic signaling pathway via death domain receptors and biological process this GO :2001237 and negative regulation of extrinsic apoptotic signaling pathway and biological process this GO :2001239 and regulation of extrinsic apoptotic signaling pathway in absence of ligand and biological process, this GO :0002020: protease binding, this GO :0002020: protease binding and also this GO :0004197: cysteine-type endopeptidase activity and also this GO :0005123: death receptor binding and also this GO :0005515: protein binding and also this GO :0008047: enzyme activator activity and also this GO :0008234: cysteine-type peptidase activity and also this GO :0032403: protein complex binding and also this GO :0035877: death effector domain binding, this GO :0004197: cysteine-type endopeptidase activity, this GO :0005123: death receptor binding, this GO :0005515: protein binding, this GO :0008047: enzyme activator activity, this GO :0008234: cysteine-type peptidase activity, this GO :0032403: protein complex binding, this GO :0035877: death effector domain binding, CASP8 and FADD-like apoptosis regulator |
| Identity | 1876 |
| Gene | CFLAR |
| Long gene name | CASP8 and FADD like apoptosis regulator |
| Synonyms gene | CASP8AP1 |
| Synonyms gene name | CASP8 and FADD-like apoptosis regulator |
| Synonyms | CASH Casper CLARP FLAME FLIP I-FLICE MRIT c-FLIP |
| Locus | 2q33, 1 |
| Discovery year | 1999-05-07 |
| GenBank acession | AF005774 |
| Entrez gene record | 8837 |
| Pubmed identfication | 9208847 9217161 |
| RefSeq identity | NM_003879 |
| Classification | Endogenous ligands Death effector domain containing Death inducing signaling complex |
| Havana BLAST/BLAT | OTTHUMG00000132819 |
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