| Also known as | HSPB1 / HSP27 |
| Other names | 28 kDa heat shock protein, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, HSP 27, HSP 27, Heat shock 27 kDa protein, Heat shock protein beta-1, SRP27, Stress-responsive protein 27, epididymis secretory protein Li 102, estrogen-regulated 24 kDa protein, heat shock 27 kDa protein, heat shock 27kD protein 1, heat shock 27kDa protein 1, heat shock protein beta-1, stress-responsive protein 27, heat shock protein beta-1 |
| Category | Antibodies |
| Subcategory | Mnoclonal antibodies |
| Gene name | HSPB1 |
| Gene name synonims | HSP27 |
| Other gene names | CMT2F, HEL-S-102, HMN2B, HS, HSP 27, HSP27, HSP27, HSP28, HSP28, HSPB1, Hsp25, HspB1, SRP27, SRP27, 76067, HSPB1 |
| Clonality | Monoclonal |
| Immunoglobulin isotype | IgG2b, k |
| Clone | 5D12-A12 |
| Host organism | Mouse (Mus musculus) |
| Species reactivity | Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Human (Homo sapiens) |
| Specificity and cross-reactivity | Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, There is no cross-reactivity to alphaBcrystallin, This particular antibody might cross react with speacies outside of the listed ones, Detects a 27kD protein corresponding to the molecular mass of hsp27 on SDS PAGE immunoblots |
| Purification method | Protein G Purified |
| Form/Appearance | 0, 50% glycerol, pH 7, 09% sodium azide, 4, PBS |
| Concentration | 1 miligram per 1 mililiter |
| Storage and shipping | For long term storage aliquot and keep the antibody at -20 degrees Celsius, Freeze-thaw cycles should be avoided in order to retain the activity of the antibody, Store at +4 degrees Celsius for short term storage |
| Tested applications: | ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB), Immunohistochemistry (IHC - Paraffin) |
| Properties | Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Human proteins, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine, , (Homo sapiens, C, C for long term storage and for short term at + 5°, Homo sapiens sapiens), If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24°, humans , ssp |
| About | Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Monoclonals of this antigen are available in different clones |
| Test | A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Latin name | Mus musculus |
| Gene target | Monoclonal [ 5D12-A12] (IgG2b, k) HSPB1 / HSP27 |
| Short name | Mouse Monoclonal [clone 5D12-A12] (IgG2b, k) HSPB1 / HSP27 Antibody |
| Technique | Monoclonals or monoclonal antibodies, antibodies against human proteins, antibodies for, mouses, Antibody |
| Isotype | IgG2b |
| Species | Humans, Mouses, Human |
| Alternative name | sapiens heat shock 27kDa protein 1 / HSP27 (Antibody to), Mouse monoclonal [clonality 5D12-A12] (IgG2b, k) to H |
| Alternative technique | antibodies |
| Alternative to gene target | CMT2F and HEL-S-102 and HMN2B and HS, HSPB1 and IDBG-23058 and ENSG00000106211 and 3315, HSPB1 and IDBG-640144 and ENSBTAG00000011969 and 516099, Hspb1 and IDBG-204151 and ENSMUSG00000004951 and 15507, nuclei, poly(A) RNA binding, this GO :0000502 and proteasome complex and cellular component this GO :0001895 and retina homeostasis and biological process this GO :0005080 and protein kinase C binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005622 and intracellular and cellular component this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005819 and spindle and cellular component this GO :0005829 and cytosol and cellular component this GO :0005856 and cytoskeleton and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006446 and regulation of translational initiation and biological process this GO :0006469 and negative regulation of protein kinase activity and biological process this GO :0006928 and cellular component movement and biological process this GO :0006986 and response to unfolded protein and biological process this GO :0008219 and cell death and biological process this GO :0008426 and protein kinase C inhibitor activity and molecular function this GO :0009615 and response to virus and biological process this GO :0010467 and gene expression and biological process this GO :0016070 and RNA metabolic process and biological process this GO :0016071 and mRNA metabolic process and biological process this GO :0019901 and protein kinase binding and molecular function this GO :0030018 and Z disc and cellular component this GO :0032731 and positive regulation of interleukin-1 beta production and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0035924 and cellular response to vascular endothelial growth factor stimulus and biological process this GO :0038033 and positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway and biological process this GO :0042535 and positive regulation of tumor necrosis factor biosynthetic process and biological process this GO :0042802 and identical protein binding and molecular function this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043122 and regulation of I-kappaB kinase/NF-kappaB signaling and biological process this GO :0043130 and ubiquitin binding and molecular function this GO :0043292 and contractile fiber and cellular component this GO :0043536 and positive regulation of blood vessel endothelial cell migration and biological process this GO :0044822 and poly(A) RNA binding and molecular function this GO :0045766 and positive regulation of angiogenesis and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0071901 and negative regulation of protein serine/threonine kinase activity and biological process this GO :1902176 and negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway and biological process this GO :2001028 and positive regulation of endothelial cell chemotaxis and biological process this GO :2001234 and negative regulation of apoptotic signaling pathway and biological process, this GO :0005080: protein kinase C binding, this GO :0005080: protein kinase C binding and also this GO :0005515: protein binding and also this GO :0008426: protein kinase C inhibitor activity and also this GO :0019901: protein kinase binding and also this GO :0042802: identical protein binding and also this GO :0043130: ubiquitin binding and also this GO :0044822: poly(A) RNA binding, this GO :0005515: protein binding, this GO :0008426: protein kinase C inhibitor activity, this GO :0019901: protein kinase binding, this GO :0042802: identical protein binding, this GO :0043130: ubiquitin binding, this GO :0044822: poly(A) RNA binding, 76067 and Hsp25 and HSP27 and HSP28 and SRP27, heat shock 27kDa protein 1 |
| Identity | 5246 |
| Gene | HSPB1 |
| Long gene name | heat shock protein family B (small) member 1 |
| Synonyms gene name | heat shock 27kD protein 1 heat shock 27kDa protein 1 |
| Synonyms | HSP27 HSP28 Hs, 76067 Hsp25 CMT2F |
| Locus | 7q11, 23 |
| Discovery year | 1991-07-09 |
| GenBank acession | X54079 |
| Entrez gene record | 3315 |
| Pubmed identfication | 2243808 9344682 |
| Classification | Small heat shock proteins |
| Havana BLAST/BLAT | OTTHUMG00000023228 |
| Locus Specific Databases | Inherited Peripheral Neuropathies Mutation Database LRG_248 |
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