| Also known as | ERCC3 / XPB |
| Other names | BTF2 p89, BTF2 p89, Basic transcription factor 2 89 kDa subunit, DNA excision repair protein ERCC-3, DNA excision repair protein ERCC-3, DNA repair helicase, DNA repair protein complementing XP-B cells, DNA repair protein complementing XP-B cells, TFIIH 89 kDa subunit, TFIIH 89 kDa subunit, TFIIH basal transcription factor complex 89 kDa subunit, TFIIH basal transcription factor complex 89 kDa subunit, TFIIH basal transcription factor complex helicase XPB subunit, TFIIH basal transcription factor complex helicase XPB subunit, TFIIH p89, TFIIH p89, Xeroderma pigmentosum group B-complementing protein, basic transcription factor 2 89 kDa subunit, complementation group 3, complementation group B, excision repair cross-complementation group 3, excision repair cross-complementing rodent repair deficiency, xeroderma pigmentosum, xeroderma pigmentosum group B-complementing protein, TFIIH basal transcription factor complex helicase XPB subunit isoform a |
| Category | Antibodies |
| Subcategory | Mnoclonal antibodies |
| Gene name | ERCC3 |
| Gene name synonims | XPB |
| Other gene names | BTF2, BTF2 p89, ERCC3, GTF2H, RAD25, TFIIH, TFIIH 89 kDa subunit, TFIIH p89, XPB, XPB, XPBC, ERCC3 |
| Clonality | Monoclonal |
| Immunoglobulin isotype | IgG2b |
| Clone | 3G4 |
| Host organism | Mouse (Mus musculus) |
| Species reactivity | Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Human (Homo sapiens) |
| Specificity and cross-reactivity | Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, This particular antibody might cross react with speacies outside of the listed ones, Recognizes an epitope located between aa242-261 of ERCC-3 (largest subunit of TFIIH) |
| Purification method | Protein G Purified |
| Form/Appearance | pH 7, 1 M Tris-HCl, 5 |
| Concentration | 1 miligram per 1 mililiter |
| Storage and shipping | Cycles of freezing/defrosting can damage the peptide chains of the antibodies thus reducing the activity, Short term storage of the antibody can be done at +4 degrees Celsius, keep the antibody at -20 degrees Celsius, For long term storage |
| Tested applications: | ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB), Immunohistochemistry (IHC - Paraffin) |
| Properties | Depending on the epitopes used human ELISA kits can be cross reactive to many other species, Human proteins, Mainly analyzed are human serum, Modern , cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, plasma, primarily , saliva, urine, , (Homo sapiens, C, C for long term storage and for short term at + 5°, Homo sapiens sapiens), If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24°, humans , ssp |
| About | Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Monoclonals of this antigen are available in different clones |
| Test | A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Latin name | Mus musculus |
| Gene target | Monoclonal [ 3G4] (IgG2b) ERCC3 / XPB |
| Short name | Mouse Monoclonal [clone 3G4] (IgG2b) ERCC3 / XPB Antibody |
| Technique | Monoclonals or monoclonal antibodies, antibodies against human proteins, antibodies for, mouses, Antibody |
| Isotype | IgG2b |
| Species | Humans, Mouses, Human |
| Alternative name | complementation family 3 / XPB (Antibody to), sapiens excision repair cross-complementing rodent repair deficiency, Mouse monoclonal [clonality 3G4] (IgG2b) to H |
| Alternative technique | antibodies |
| Alternative to gene target | BTF2 and GTF2H and RAD25 and TFIIH and XPB, DNA damage removal and biological process this GO :0001666 and response to hypoxia and biological process this GO :0003677 and DNA binding and molecular function this GO :0003684 and damaged DNA binding and molecular function this GO :0004003 and ATP-dependent DNA helicase activity and molecular function this GO :0004672 and protein kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005525 and GTP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005675 and holo TFIIH complex and cellular component this GO :0006200 and ATP catabolic process and biological process this GO :0006265 and DNA topological change and biological process this GO :0006281 and DNA repair and biological process this GO :0006283 and transcription-coupled nucleotide-excision repair and biological process this GO :0006289 and nucleotide-excision repair and biological process this GO :0006360 and transcription from RNA polymerase I promoter and biological process this GO :0006361 and transcription initiation from RNA polymerase I promoter and biological process this GO :0006362 and transcription elongation from RNA polymerase I promoter and biological process this GO :0006363 and termination of RNA polymerase I transcription and biological process this GO :0006366 and transcription from RNA polymerase II promoter and biological process this GO :0006367 and transcription initiation from RNA polymerase II promoter and biological process this GO :0006368 and transcription elongation from RNA polymerase II promoter and biological process this GO :0006370 and 7-methylguanosine mRNA capping and biological process this GO :0006468 and protein phosphorylation and biological process this GO :0006915 and apoptotic process and biological process this GO :0006979 and response to oxidative stress and biological process this GO :0008022 and protein C-terminus binding and molecular function this GO :0008094 and DNA-dependent ATPase activity and molecular function this GO :0008104 and protein localization and biological process this GO :0008134 and transcription factor binding and molecular function this GO :0008353 and RNA polymerase II carboxy-terminal domain kinase activity and molecular function this GO :0009411 and response to UV and biological process this GO :0009650 and UV protection and biological process this GO :0010467 and gene expression and biological process this GO :0016032 and viral process and biological process this GO :0016787 and hydrolase activity and molecular function this GO :0016887 and ATPase activity and molecular function this GO :0032508 and DNA duplex unwinding and biological process this GO :0032564 and dATP binding and molecular function this GO :0033683 and nucleotide-excision repair, DNA duplex unwinding and biological process this GO :0000718 and nucleotide-excision repair, DNA incision and biological process this GO :0035315 and hair cell differentiation and biological process this GO :0042277 and peptide binding and molecular function this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0043138 and 3'-5' DNA helicase activity and molecular function this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0047485 and protein N-terminus binding and molecular function this GO :0050434 and positive regulation of viral transcription and biological process this GO :1901990 and regulation of mitotic cell cycle phase transition and biological process, ERCC3 and IDBG-639240 and ENSBTAG00000020777 and 507984, ERCC3 and IDBG-68963 and ENSG00000163161 and 2071, Ercc3 and IDBG-133673 and ENSMUSG00000024382 and 13872, complementation group 3, nuclei, protein N-terminus binding, this GO :0000439 and core TFIIH complex and cellular component this GO :0000717 and nucleotide-excision repair, this GO :0003677: DNA binding, this GO :0003677: DNA binding and also this GO :0003684: damaged DNA binding and also this GO :0004003: ATP-dependent DNA helicase activity and also this GO :0004672: protein kinase activity and also this GO :0005515: protein binding and also this GO :0005524: ATP binding and also this GO :0005525: GTP binding and also this GO :0008022: protein C-terminus binding and also this GO :0008094: DNA-dependent ATPase activity and also this GO :0008134: transcription factor binding and also this GO :0008353: RNA polymerase II carboxy-terminal domain kinase activity and also this GO :0016787: hydrolase activity and also this GO :0016887: ATPase activity and also this GO :0032564: dATP binding and also this GO :0042277: peptide binding and also this GO :0043138: 3'-5' DNA helicase activity and also this GO :0047485: protein N-terminus binding, this GO :0003684: damaged DNA binding, this GO :0004003: ATP-dependent DNA helicase activity, this GO :0004672: protein kinase activity, this GO :0005515: protein binding, this GO :0005524: ATP binding, this GO :0005525: GTP binding, this GO :0008022: protein C-terminus binding, this GO :0008094: DNA-dependent ATPase activity, this GO :0008134: transcription factor binding, this GO :0008353: RNA polymerase II carboxy-terminal domain kinase activity, this GO :0016787: hydrolase activity, this GO :0016887: ATPase activity, this GO :0032564: dATP binding, this GO :0042277: peptide binding, this GO :0043138: 3'-5' DNA helicase activity, this GO :0047485: protein N-terminus binding, excision repair cross-complementing rodent repair deficiency |
| Identity | 3435 |
| Gene | ERCC3 |
| Long gene name | ERCC excision repair 3, TFIIH core complex helicase subunit |
| Synonyms gene name | complementation group 3 excision repair cross-complementation group 3 , excision repair cross-complementing rodent repair deficiency |
| Synonyms | XPB BTF2 RAD25 TFIIH GTF2H |
| Synonyms name | xeroderma pigmentosum group B complementing |
| Locus | 2q14, 3 |
| Discovery year | 2001-06-22 |
| GenBank acession | M31899 |
| Entrez gene record | 2071 |
| Pubmed identfication | 8202161 |
| RefSeq identity | NM_000122 |
| Classification | Xeroderma pigmentosum complementation groups General transcription factors ERCC excision repair associated DNA helicases |
| Havana BLAST/BLAT | OTTHUMG00000131530 |
| Locus Specific Databases | LRG_462 |
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