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MPZL2/EVA1 Antibody, FITC Conjugated

MPZL2/EVA1 Antibody, FITC Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name FITC Conjugated, MPZL2/EVA1 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenMPZL2/EVA1
SpecificityThis is a highly specific antibody against MPZL2/EVA1
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Subcellular locationExtracellular
SourceKLH conjugated synthetic peptide derived from human MPZL2/EVA1
Tested applications IF(IHC-P), FCM
Recommended dilutions IF(IHC-P)(1:50-200), FCM(1:20-100)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Epithelial V-like antigen (EVA) is expressed in thymus epithelium and strongly downregulated by thymocyte developmental progression, It is highly homologous to the myelin protein zero and, Its capacity to mediate cell adhesion through a homophilic interaction and its selective regulation by T cell maturation might imply the participation of EVA in the earliest phases of thymus organogenesis, Jul 2008], The protein bears a characteristic V-type domain and two potential N-glycosylation sites in the extracellular domain, This gene is expressed in the thymus and in several epithelial structures early in embryogenesis, Two transcript variants encoding the same protein have been found for this gene, [provided by RefSeq, a putative serine phosphorylation site for casein kinase 2 is also present in the cytoplasmic tail, in thymus-derived epithelial cell lines, is poorly soluble in nonionic detergents, strongly suggesting an association to the cytoskeleton, Thymus development depends on a complex series of interactions between thymocytes and the stromal component of the organ
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms EVA
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetMPZL2/EVA1 Conjugated
Short name FITC Conjugated, MPZL2/EVA1 Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, myelin protein zero-like 2/EVA1 (Antibody to)
Alternative techniqueantibodies
Alternative to gene target EVA and EVA1, MPZL2 and IDBG-631254 and ENSBTAG00000033510 and 540423, MPZL2 and IDBG-73587 and ENSG00000149573 and 10205, Mpzl2 and IDBG-159117 and ENSMUSG00000032092 and 14012, Plasma membranes, protein binding, this GO :0005515 and protein binding and molecular function this GO :0005856 and cytoskeleton and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0007156 and homophilic cell adhesion and biological process this GO :0009653 and anatomical structure morphogenesis and biological process this GO :0016021 and integral component of membrane and cellular component this GO :0016337 and single organismal cell-cell adhesion and biological process this GO :0033077 and T cell differentiation in thymus and biological process, this GO :0005515: protein binding, this GO :0005515: protein binding, myelin protein zero-like 2
Identity 3496
Gene MPZL2
Long gene name myelin protein zero like 2
Synonyms gene EVA1
Synonyms gene name epithelial V-like antigen 1
Locus 11q24
Discovery year 1999-11-22
GenBank acession AF275945
Entrez gene record 10205
Pubmed identfication 9585423
RefSeq identity NM_005797
Classification V-set domain containing
Havana BLAST/BLAT OTTHUMG00000166967

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