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MYPT1 (Thr853) Antibody, FITC Conjugated

MYPT1 (Thr853) Antibody, FITC Conjugated size: 0.1ml 350

Price 350
Size 0.1ml
Long name FITC Conjugated, MYPT1 (Thr853) Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenMYPT1 Thr853
Specificity This phosphorylation site is homologous to that of Thr852 in Mouse and Thr855 in Rat, This is a highly specific antibody against MYPT1 Thr853
ModificationPhosphorylation
Modification siteThr853
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range830-880/1030
Subcellular locationCytoplasm
SourceKLH conjugated synthetic phosphopeptide derived from human around the phosphorylation site of Thr853
Gene ID number4659
Swiss ProtO14974
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background As part of the PPP1C complex, Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity, Mediates binding to myosin, involved in dephosphorylation of PLK1, Key regulator of protein phosphatase 1C (PPP1C)
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms MBS M130
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetMYPT1 (Thr853) Conjugated
Short name FITC Conjugated, MYPT1 (Thr853) Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, MYPT1 (Thr853) (Antibody to)
Alternative techniqueantibodies
Identity 7618
Gene PPP1R12A
Long gene name protein phosphatase 1 regulatory subunit 12A
Synonyms gene MYPT1
Synonyms gene name protein phosphatase 1, regulatory (inhibitor) subunit 12A protein phosphatase 1, regulatory subunit 12A
Synonyms name myosin phosphatase-targeting subunit 1 myosin binding subunit
Locus 12q21, 2-q21, 31
Discovery year 1997-12-23
GenBank acession D87930
Entrez gene record 4659
Pubmed identfication 9286714
RefSeq identity NM_002480
Classification Ankyrin repeat domain containing Protein phosphatase 1 regulatory subunits
Havana BLAST/BLAT OTTHUMG00000170100

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