| Long name | Cy3 Conjugated, NCF1/p47 phox(Ser359) Antibody |
| Category | Conjugated Primary Antibodies |
| Conjugation | cy3 conjugation kit |
| Host Organism | Rabbit (Oryctolagus cuniculus) |
| Target Antigen | NCF1/p47 phox Ser359 |
| Specificity | This is a highly specific antibody against NCF1/p47 phox Ser359 |
| Modification | Phosphorylation |
| Modification site | Ser359 |
| Clonality | Polyclonal |
| Clone | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Source | KLH conjugated synthetic phosphopeptide derived from human NCF1 around the phosphorylation site of Ser359 |
| Gene ID number | 653361 |
| Tested applications | IF(IHC-P) |
| Recommended dilutions | IF(IHC-P)(1:50-200) |
| Crossreactivity | Rat, Human |
| Crossreactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background | Both p47-phox and p67-phox contain two Src homology 3 (SH3) domains, During activation of the NADPH oxidase, The C-terminal SH3 domain of p67-phox has been shown to interact with the proline-rich domain of p47-phox, The cytosolic proteins p47-phox and p67-phox, also designated neutrophil cytosol factor (NCF)1 and NCF2, are required for activation of the superoxide-producing NADPH oxidase in neutrophils and other phagocytic cells, p47-phox and p67-phox migrate to the plasma membrane where they associate with cytochrome b558 and the small G protein Rac to form the functional enzyme complex, respectively, suggesting that p47-phox may faciliate the transport of p67-phox to the membrane, The heredity chronic granulomatous disease (CGF) has been linked to mutations in p47-phox and p67-phox |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission | 512, 550nm/570, 615nm |
| Synonyms | p47phox NOXO2 NCF1A SH3PXD1A |
| Properties | 1 of the NCF1/p47 phox(Ser359) Antibody, Conjugated can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC, Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser, This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target | NCF1/p47 phox(Ser359) Conjugated |
| Short name | Conjugated, NCF1/p47 phox(Ser359) Antibody |
| Technique | antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label | Cy3 |
| Alternative name | cyanine 3 coupled, neutrophil cytosolic factor 1/p47 phox(Ser359) (Antibody to) |
| Alternative technique | antibodies |
| Alternative to gene target | NCF1 and IDBG-21570 and ENSG00000158517 and 653361, NCF1 and IDBG-639883 and ENSBTAG00000003305 and 281345, Ncf1 and IDBG-202592 and ENSMUSG00000015950 and 17969, Plasma membranes, TAP-dependent and biological process this GO :0002679 and respiratory burst involved in defense response and biological process this GO :0005515 and protein binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005791 and rough endoplasmic reticulum and cellular component this GO :0005794 and this GO lgi apparatus and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006612 and protein targeting to membrane and biological process this GO :0006691 and leukotriene metabolic process and biological process this GO :0006801 and superoxide metabolic process and biological process this GO :0006915 and apoptotic process and biological process this GO :0006954 and inflammatory response and biological process this GO :0006968 and cellular defense response and biological process this GO :0008283 and cell proliferation and biological process this GO :0009055 and electron carrier activity and molecular function this GO :0009617 and response to bacterium and biological process this GO :0016020 and membrane and cellular component this GO :0016175 and superoxide-generating NADPH oxidase activity and molecular function this GO :0017124 and SH3 domain binding and molecular function this GO :0019898 and extrinsic component of membrane and cellular component this GO :0030425 and dendrite and cellular component this GO :0032010 and pha this GO lysosome and cellular component this GO :0035091 and phosphatidylinositol binding and molecular function this GO :0042554 and superoxide anion generation and biological process this GO :0042590 and antigen processing and presentation of exogenous peptide antigen via MHC class I and biological process this GO :0042742 and defense response to bacterium and biological process this GO :0043020 and NADPH oxidase complex and cellular component this GO :0043025 and neuronal cell body and cellular component this GO :0043325 and phosphatidylinositol-3, phosphatidylinositol-3, this GO :0001878 and response to yeast and biological process this GO :0001909 and leukocyte mediated cytotoxicity and biological process this GO :0002474 and antigen processing and presentation of peptide antigen via MHC class I and biological process this GO :0002479 and antigen processing and presentation of exogenous peptide antigen via MHC class I, this GO :0005515: protein binding, this GO :0005515: protein binding and also this GO :0009055: electron carrier activity and also this GO :0016175: superoxide-generating NADPH oxidase activity and also this GO :0017124: SH3 domain binding and also this GO :0035091: phosphatidylinositol binding and also this GO :0043325: phosphatidylinositol-3, this GO :0009055: electron carrier activity, this GO :0016175: superoxide-generating NADPH oxidase activity, this GO :0017124: SH3 domain binding, this GO :0035091: phosphatidylinositol binding, this GO :0043325: phosphatidylinositol-3, 4-bisphosphate binding, 4-bisphosphate binding, 4-bisphosphate binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0045730 and respiratory burst and biological process this GO :0045986 and negative regulation of smooth muscle contraction and biological process this GO :0050665 and hydrogen peroxide biosynthetic process and biological process this GO :0050830 and defense response to Gram-positive bacterium and biological process this GO :0050832 and defense response to fungus and biological process this GO :0051701 and interaction with host and biological process this GO :0055114 and oxidation-reduction process and biological process this GO :0070946 and neutrophil mediated killing of gram-positive bacterium and biological process this GO :0070947 and neutrophil mediated killing of fungus and biological process this GO :0090382 and pha this GO some maturation and biological process, neutrophil cytosolic factor 1 |
| Identity | 7660 |
| Gene | NCF1 |
| Long gene name | neutrophil cytosolic factor 1 |
| Synonyms gene name | autosomal 1) , chronic granulomatous disease, neutrophil cytosolic factor 1 (47kD |
| Synonyms name | NADPH oxidase organizer 2 chronic granulomatous disease, autosomal 1 |
| Locus | 7q11, 23 |
| Discovery year | 1989-05-25 |
| GenBank acession | M25665 |
| Entrez gene record | 653361 |
| RefSeq identity | NM_000265 |
| Havana BLAST/BLAT | OTTHUMG00000149965 |
| Locus Specific Databases | NCF1base: Mutation registry for autosomal recessive chronic granulomatous disease (CGD), deficiency of p47phox LRG_87 |
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