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Nck1/NCK alpha Antibody, Biotin Conjugated

Nck1/NCK alpha Antibody, Biotin Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name Biotin Conjugated, Nck1/NCK alpha Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenNck1/NCK alpha
SpecificityThis is a highly specific antibody against Nck1/NCK alpha
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human Nck2
Gene ID number4690
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background It is a common target for the action of different surface receptors, Like other SH2 containing proteins, Nck is associated with tyrosine autophosphorylated EGF or PDGF receptors via its SH2 domain, Nck is phosphorylated on tyrosine, Nck is widely expressed in various tissues and in cell lines from human, Overexpression of Nck leads to transformation of NIH 3T3 cells, Ras GTPase activating protein, and phospholipase Cg, and rat origins, and threonine residues in response to stimulation of EGF and PDGF in A431 and NIH 3T3 cells respectively, encoding one SH2 and three SH3 domains, murine, oncogenic protein, phosphatidylinositol 3 kinase, serine, the Src homology motifs found in nonreceptor tyrosine kinases, Nck is a highly conserved
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms NCKalpha
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetNck1/NCK alpha Conjugated
Short name Conjugated, Nck1/NCK alpha Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, NCK adaptor protein 1/NCK a (Antibody to)
Alternative techniqueantibodies
Identity 7664
Gene NCK1
Long gene name NCK adaptor protein 1
Synonyms gene NCK
Locus 3q22, 3
Discovery year 1998-11-04
GenBank acession X17576
Entrez gene record 4690
Pubmed identfication 7806213 9737977
RefSeq identity NM_006153
Classification SH2 domain containing
Havana BLAST/BLAT OTTHUMG00000159781

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