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Nck1/NCK alpha Antibody, FITC Conjugated

Nck1/NCK alpha Antibody, FITC Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, Nck1/NCK alpha Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenNck1/NCK alpha
SpecificityThis is a highly specific antibody against Nck1/NCK alpha
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human Nck2
Gene ID number4690
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background It is a common target for the action of different surface receptors, Like other SH2 containing proteins, Nck is associated with tyrosine autophosphorylated EGF or PDGF receptors via its SH2 domain, Nck is phosphorylated on tyrosine, Nck is widely expressed in various tissues and in cell lines from human, Overexpression of Nck leads to transformation of NIH 3T3 cells, Ras GTPase activating protein, and phospholipase Cg, and rat origins, and threonine residues in response to stimulation of EGF and PDGF in A431 and NIH 3T3 cells respectively, encoding one SH2 and three SH3 domains, murine, oncogenic protein, phosphatidylinositol 3 kinase, serine, the Src homology motifs found in nonreceptor tyrosine kinases, Nck is a highly conserved
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms NCKalpha
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetNck1/NCK alpha Conjugated
Short name FITC Conjugated, Nck1/NCK alpha Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, NCK adaptor protein 1/NCK a (Antibody to)
Alternative techniqueantibodies
Identity 7664
Gene NCK1
Long gene name NCK adaptor protein 1
Synonyms gene NCK
Locus 3q22, 3
Discovery year 1998-11-04
GenBank acession X17576
Entrez gene record 4690
Pubmed identfication 7806213 9737977
RefSeq identity NM_006153
Classification SH2 domain containing
Havana BLAST/BLAT OTTHUMG00000159781

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