Name : NCK2 Antibody, FITC Conjugated
Supplier : BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price :333
SKU : GEN9349741374
| Long name | FITC Conjugated, NCK2 Polyclonal Antibody |
| Category | Conjugated Primary Antibodies |
| Conjugation | Anti-FITC Antibody |
| Host Organism | Rabbit (Oryctolagus cuniculus) |
| Target Antigen | NCK2 |
| Specificity | This is a highly specific antibody against NCK2 |
| Modification | Unmodified |
| Modification site | None |
| Clonality | Polyclonal |
| Clone | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Source | KLH conjugated synthetic peptide derived from human NCK2 |
| Gene ID number | 8440 |
| Tested applications | IF(IHC-P) |
| Recommended dilutions | IF(IHC-P)(1:50-200) |
| Crossreactivity | Mouse, Rat, Human |
| Crossreactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background | Alternate transcriptional splice variants, It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization, The protein contains three SH3 domains and one SH2 domain, The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases, [provided by RefSeq], encoding different isoforms, have been characterized, This gene encodes a member of the NCK family of adaptor proteins |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission | 494nm/518nm |
| Synonyms | NCKbeta |
| Properties | Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target | NCK2 Conjugated |
| Short name | FITC Conjugated, NCK2 Antibody |
| Technique | Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label | FITC |
| Alternative name | fluorecein coupled, NCK adaptor protein 2 (Antibody to) |
| Alternative technique | antibodies |
| Alternative to gene target | Cytoplasm, GRB4 and NCKbeta, NCK2 and IDBG-634552 and ENSBTAG00000015205 and 526430, NCK2 and IDBG-64634 and ENSG00000071051 and 8440, Nck2 and IDBG-149650 and ENSMUSG00000066877 and 17974, receptor signaling complex scaffold activity, this GO :0005515 and protein binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005783 and endoplasmic reticulum and cellular component this GO :0005829 and cytosol and cellular component this GO :0006417 and regulation of translation and biological process this GO :0007015 and actin filament organization and biological process this GO :0007165 and signal transduction and biological process this GO :0007172 and signal complex assembly and biological process this GO :0007173 and epidermal growth factor receptor signaling pathway and biological process this GO :0007176 and regulation of epidermal growth factor-activated receptor activity and biological process this GO :0007411 and axon guidance and biological process this GO :0008093 and cytoskeletal adaptor activity and molecular function this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0012506 and vesicle membrane and cellular component this GO :0016477 and cell migration and biological process this GO :0030032 and lamellipodium assembly and biological process this GO :0030159 and receptor signaling complex scaffold activity and molecular function this GO :0030838 and positive regulation of actin filament polymerization and biological process this GO :0042102 and positive regulation of T cell proliferation and biological process this GO :0042110 and T cell activation and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process, this GO :0005515: protein binding, this GO :0005515: protein binding and also this GO :0008093: cytoskeletal adaptor activity and also this GO :0030159: receptor signaling complex scaffold activity, this GO :0008093: cytoskeletal adaptor activity, this GO :0030159: receptor signaling complex scaffold activity, NCK adaptor protein 2 |
| Identity | 7665 |
| Gene | NCK2 |
| Long gene name | NCK adaptor protein 2 |
| Locus | 2q12, 2 |
| Discovery year | 1998-10-29 |
| GenBank acession | AF043119 |
| Entrez gene record | 8440 |
| Pubmed identfication | 9737977 16752908 |
| RefSeq identity | NM_003581 |
| Classification | SH2 domain containing |
| Havana BLAST/BLAT | OTTHUMG00000153116 |