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NEDD8 Antibody, Biotin Conjugated

NEDD8 Antibody, Biotin Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name Biotin Conjugated, NEDD8 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenNEDD8
SpecificityThis is a highly specific antibody against NEDD8
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human NEDD8
Gene ID number4738
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Apg12 and Hub1, Cullins are subunits of an E3 ubiquitin ligase complex called the Skp1 /Cul1/Cdc53 F box (SCF), Examples of UBLs are SUMO, In contrast to UBLs, NEDD8 shows 80% homology to ubiquitin, Proteins of the second class include Parkin, RAD23 and DSK2, Rub1 (also called Nedd8), Rub1 and the corresponding human homolog NEDD8 are activated by the E1 ubiquitin activating enzyme UBA2, Rub1 modification may regulate SCF function or localization, The SCF promotes transfer of ubiquitin from a ubiquitin conjugating enzyme (E2) to the target protein, The best known targets of Rub1 modification are members of the cullin family, These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other, UDPs are not proteolytically processed or conjugated to other proteins, are designated ubiquitin domain proteins (UDPs), that forms isopeptide linkages between thio esters, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin, Ubiquitin like proteins fall into two classes: the first class
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms Nedd-8
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetNEDD8 Conjugated
Short name Conjugated, NEDD8 Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, NEDD8 (Antibody to)
Alternative techniqueantibodies
Identity 7732
Gene NEDD8
Long gene name developmentally down-regulated 8 , neural precursor cell expressed
Locus 14q12
Discovery year 1994-01-21
GenBank acession D23662
Entrez gene record 4738
Pubmed identfication 9353319
RefSeq identity NM_006156
Havana BLAST/BLAT OTTHUMG00000029325

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