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NEK2 Antibody, FITC Conjugated

NEK2 Antibody, FITC Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, NEK2 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenNEK2
SpecificityThis is a highly specific antibody against NEK2
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range5-55/445
Subcellular location Nucleus, Cytoplasm
SourceKLH conjugated synthetic peptide derived from human NEK2
Gene ID number4751
Swiss ProtP51955
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background CEP25 and NINL, Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome, Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC2 and MAD2L1, Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells, Isoform 2, NECAB3 and NPM1, Phosphorylates: PPP1CC, Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2, Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC8, SGOL1, does not, resulting in their displacement from the centrosomes, which is not present in the nucleolus, Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms NLK1 NEK2A RP67 PPP1R111
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetNEK2 Conjugated
Short name FITC Conjugated, NEK2 Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, NEK2 (Antibody to)
Alternative techniqueantibodies
Identity 7745
Gene NEK2
Long gene name NIMA related kinase 2
Synonyms gene name NIMA (never in mitosis gene a)-related kinase 2
Synonyms name HsPK 21 protein phosphatase 1, regulatory subunit 111
Locus 1q32, 3
Discovery year 1998-08-26
GenBank acession U11050
Entrez gene record 4751
Pubmed identfication 8274451 24043777
RefSeq identity NM_002497
Classification Protein phosphatase 1 regulatory subunits
Havana BLAST/BLAT OTTHUMG00000037121

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