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NFIA/NF-1 Antibody, Biotin Conjugated

NFIA/NF-1 Antibody, Biotin Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name Biotin Conjugated, NFIA/NF-1 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenNFIA/NF-1
SpecificityThis is a highly specific antibody against NFIA/NF-1
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human NFIA/NF-1
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene, GRP 78, HSP 70, Histone H1, NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA binding domain, The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes, alpha-globin, also designated CTF, and activates transcription through a putatively novel, carboxy terminal transactivation domain, consists of a family of CCAAT box binding proteins that stimulate DNA replication and activate transcription, including that of Ha-Ras, myelin basic protein and in the Xenopus laevis vitellogenin gene promoter, proline-rich, NF-1
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms NFI-L KIAA1439
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetNFIA/NF-1 Conjugated
Short name Conjugated, NFIA/NF-1 Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, NFIA/NF-1 (Antibody to)
Alternative techniqueantibodies
Identity 7784
Gene NFIA
Long gene name nuclear factor I A
Synonyms gene name nuclear factor I/A
Locus 1p31, 3
Discovery year 1995-03-09
GenBank acession U07809
Entrez gene record 4774
Pubmed identfication 7590749
RefSeq identity NM_005595
Havana BLAST/BLAT OTTHUMG00000008618

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