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NFIA/NF-1 Antibody, FITC Conjugated

NFIA/NF-1 Antibody, FITC Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name FITC Conjugated, NFIA/NF-1 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenNFIA/NF-1
SpecificityThis is a highly specific antibody against NFIA/NF-1
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human NFIA/NF-1
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene, GRP 78, HSP 70, Histone H1, NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA binding domain, The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes, alpha-globin, also designated CTF, and activates transcription through a putatively novel, carboxy terminal transactivation domain, consists of a family of CCAAT box binding proteins that stimulate DNA replication and activate transcription, including that of Ha-Ras, myelin basic protein and in the Xenopus laevis vitellogenin gene promoter, proline-rich, NF-1
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms NFI-L KIAA1439
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetNFIA/NF-1 Conjugated
Short name FITC Conjugated, NFIA/NF-1 Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, NFIA/NF-1 (Antibody to)
Alternative techniqueantibodies
Identity 7784
Gene NFIA
Long gene name nuclear factor I A
Synonyms gene name nuclear factor I/A
Locus 1p31, 3
Discovery year 1995-03-09
GenBank acession U07809
Entrez gene record 4774
Pubmed identfication 7590749
RefSeq identity NM_005595
Havana BLAST/BLAT OTTHUMG00000008618

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