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NFKB Antibody, Biotin Conjugated

NFKB Antibody, Biotin Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name Biotin Conjugated, NFKB Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenNFKB p100/p52
Specificity The immunoge used to produce this antibody has 83% sequence similarity with NFKB p105/ p50, This is a highly specific antibody against NFKB p100/p52
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range200-250/900
Subcellular location Nucleus, Cytoplasm
SourceKLH conjugated synthetic peptide derived from human NFKB p52
Gene ID number4791
Swiss ProtQ00653
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Alternative splicing results in multiple transcript variants, Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, Dec 2013], The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function, The p100 full-length protein is co-translationally processed into a p52 active form, The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner, There is a pseudogene for this gene on chromosome 18, [provided by RefSeq, some of which may result in the formation of fusion proteins, This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB)
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms CVID10, DNA-binding factor KBF2, H2TF1, LYT-10, LYT10, Lymphocyte translocation chromosome 10 protein, NF-kB2, NFKB2, Nuclear factor NF-kappa-B p100 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, Oncogene Lyt-10, p100, p52
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetNFKB Conjugated
Short name Conjugated, NFKB Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, NFKB (Antibody to)
Alternative techniqueantibodies

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