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NFKB p65 Antibody, Biotin Conjugated

NFKB p65 Antibody, Biotin Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name Biotin Conjugated, NFKB p65 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenNFKB p65
SpecificityThis is a highly specific antibody against NFKB p65
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range50-100/551
Subcellular locationCytoplasm
SourceKLH conjugated synthetic peptide derived from human NFKBp65
Gene ID number5970
Swiss ProtQ04206
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Pig, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Associates with chromatin at the NF-kappa-B promoter region via association with DDX1, Different dimer combinations act as transcriptional activators or repressors, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, In a conventional activation pathway, NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family, NF-kappa-B heterodimeric p65-p5 and p65-c-Rel complexes are transcriptional activators, NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors, NFKB1/p15, NFKB1/p5, REL and NFKB2/p52 and the heterodimeric p65-p5 complex appears to be most abundant one, RELB, The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression, The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity, The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65, cell growth, differentiation, immunity, p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex, respectively, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus, tumorigenesis and apoptosis, NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms NFKB3, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA, Transcription factor p65, p65
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetNFKB p65 Conjugated
Short name Conjugated, NFKB p65 Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, NFKB p65 (Antibody to)
Alternative techniqueantibodies

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