| Antigen detectable with this ELISA kit | Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) |
| Species reactivity | Homo sapiens (Human) |
| Range of detection | 0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity | 065ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application | ELISA |
| Sample types tested for this ELISA kit | cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test | 4, 5 hoursours |
| Test principle | 10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1), The concentration of Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling | Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory | Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties | E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test | ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target | Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) |
| Short name | Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) ELISA kit |
| Technique | &hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host | antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name | Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NADPH oxidase 1) Enzyme-linked immunosorbent assay reagent |
| Alternative technique | elisas |
| Alternative to gene target | Cell surfaces, NADP binding, NOX1 and IDBG-633329 and ENSBTAG00000016761 and 521681, NOX1 and IDBG-79287 and ENSG00000007952 and 27035, Nox1 and IDBG-170699 and ENSMUSG00000031257 and 237038, this GO :0001525 and angiogenesis and biological process this GO :0003081 and regulation of systemic arterial blood pressure by renin-angiotensin and biological process this GO :0005515 and protein binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005768 and endosome and cellular component this GO :0005769 and early endosome and cellular component this GO :0006739 and NADP metabolic process and biological process this GO :0006801 and superoxide metabolic process and biological process this GO :0006954 and inflammatory response and biological process this GO :0007165 and signal transduction and biological process this GO :0008217 and regulation of blood pressure and biological process this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0010575 and positive regulation vascular endothelial growth factor production and biological process this GO :0015992 and proton transport and biological process this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0016175 and superoxide-generating NADPH oxidase activity and molecular function this GO :0016477 and cell migration and biological process this GO :0016491 and oxidoreductase activity and molecular function this GO :0030054 and cell junction and cellular component this GO :0030171 and voltage-gated proton channel activity and molecular function this GO :0030198 and extracellular matrix organization and biological process this GO :0034765 and regulation of ion transmembrane transport and biological process this GO :0042554 and superoxide anion generation and biological process this GO :0042743 and hydrogen peroxide metabolic process and biological process this GO :0043020 and NADPH oxidase complex and cellular component this GO :0043410 and positive regulation of MAPK cascade and biological process this GO :0045087 and innate immune response and biological process this GO :0045726 and positive regulation of integrin biosynthetic process and biological process this GO :0045730 and respiratory burst and biological process this GO :0046330 and positive regulation of JNK cascade and biological process this GO :0046872 and metal ion binding and molecular function this GO :0048365 and Rac GTPase binding and molecular function this GO :0048661 and positive regulation of smooth muscle cell proliferation and biological process this GO :0050661 and NADP binding and molecular function this GO :0051454 and intracellular pH elevation and biological process this GO :0055114 and oxidation-reduction process and biological process this GO :0071438 and invadopodium membrane and cellular component this GO :0071455 and cellular response to hyperoxia and biological process this GO :0071468 and cellular response to acidity and biological process this GO :0072592 and oxygen metabolic process and biological process this GO :1902177 and positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway and biological process, this GO :0005515: protein binding, this GO :0005515: protein binding and also this GO :0016175: superoxide-generating NADPH oxidase activity and also this GO :0016491: oxidoreductase activity and also this GO :0030171: voltage-gated proton channel activity and also this GO :0046872: metal ion binding and also this GO :0048365: Rac GTPase binding and also this GO :0050661: NADP binding, this GO :0016175: superoxide-generating NADPH oxidase activity, this GO :0016491: oxidoreductase activity, this GO :0030171: voltage-gated proton channel activity, this GO :0046872: metal ion binding, this GO :0048365: Rac GTPase binding, this GO :0050661: NADP binding, NADPH oxidase 1 |
| Identity | 7889 |
| Gene | NOX1 |
| Long gene name | NADPH oxidase 1 |
| Synonyms | NOH1 NOH-1 MOX1 GP91-2 |
| Synonyms name | mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating) NADPH oxidase homolog-1 NADPH oxidase 1 variant NOH-1L |
| Locus | Xq22, 1 |
| Discovery year | 2000-03-24 |
| GenBank acession | AF127763 |
| Entrez gene record | 27035 |
| Pubmed identfication | 10485709 10615049 |
| RefSeq identity | NM_007052 |
| Havana BLAST/BLAT | OTTHUMG00000022007 |
| Locus Specific Databases | Mental Retardation database |