| Long name | FITC Conjugated, 2/NKX2-2 Polyclonal Antibody, Nkx2 |
| Category | Conjugated Primary Antibodies |
| Conjugation | Anti-FITC Antibody |
| Host Organism | Rabbit (Oryctolagus cuniculus) |
| Target Antigen | 2/NKX2-2, Nkx2 |
| Specificity | 2/NKX2-2, This is a highly specific antibody against Nkx2 |
| Modification | Unmodified |
| Modification site | None |
| Clonality | Polyclonal |
| Clone | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Source | 2, KLH conjugated synthetic peptide derived from human Nkx2 |
| Tested applications | IF(IHC-P) |
| Recommended dilutions | IF(IHC-P)(1:50-200) |
| Crossreactivity | Mouse, Rat, Human |
| Crossreactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background | During neural development, Homozygous null mutations of the Nkx-2, In the pancreas, Nkx-2, Nkx-2, Nkx-2, Specifically, b and pancreatic polypeptide (PP) cells, but not in d cells, heart and pancreas, including Nkx-2, including brain, including diabetes and cancer, sonic hedgehog (Shh) is known to control cell fate and mitogenesis, the Nkx-2, which is correlated with Shh dose-dependent expression of several genes, which produce insulin, which suggests that Nkx-2, 1, 2 and Nkx-2, 2 expression is required for differentiation of pancreatic b cells, 2 gene in mice lead to severe hyperglycemia and death shortly after birth, 2 is expressed in a, 2 may be an important therapeutic target for pancreatic diseases, 2 protein is responsible for directing ventral neuronal patterning in response to graded Shh signaling, 9, Members of the NK-2 family of homeodomain proteins are key regulators of growth and development in several tissues |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission | 494nm/518nm |
| Synonyms | NKX2, 2 |
| Properties | Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target | 2/NKX2-2 Conjugated, Nkx2 |
| Short name | FITC Conjugated, 2/NKX2-2 Antibody, Nkx2 |
| Technique | Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label | FITC |
| Alternative name | fluorecein coupled, 2/NK2 homeobox 2 (Antibody to), Nkx2 |
| Alternative technique | antibodies |
| Alternative to gene target | BT, DNA-templated and biological process this GO :0006355 and regulation of transcription, DNA-templated and biological process this GO :0007224 and smoothened signaling pathway and biological process this GO :0007399 and nervous system development and biological process this GO :0007420 and brain development and biological process this GO :0008134 and transcription factor binding and molecular function this GO :0009749 and response to glucose and biological process this GO :0014003 and oli this GO dendrocyte development and biological process this GO :0014070 and response to organic cyclic compound and biological process this GO :0021522 and spinal cord motor neuron differentiation and biological process this GO :0021529 and spinal cord oli this GO dendrocyte cell differentiation and biological process this GO :0021530 and spinal cord oli this GO dendrocyte cell fate specification and biological process this GO :0021554 and optic nerve development and biological process this GO :0030154 and cell differentiation and biological process this GO :0031018 and endocrine pancreas development and biological process this GO :0032570 and response to progesterone and biological process this GO :0043565 and sequence-specific DNA binding and molecular function this GO :0045595 and regulation of cell differentiation and biological process this GO :0045597 and positive regulation of cell differentiation and biological process this GO :0045665 and negative regulation of neuron differentiation and biological process this GO :0045666 and positive regulation of neuron differentiation and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0048468 and cell development and biological process this GO :0048565 and digestive tract development and biological process this GO :0048665 and neuron fate specification and biological process this GO :0048708 and astrocyte differentiation and biological process this GO :0048714 and positive regulation of oli this GO dendrocyte differentiation and biological process this GO :0051091 and positive regulation of sequence-specific DNA binding transcription factor activity and biological process this GO :0060580 and ventral spinal cord interneuron fate determination and biological process, NKX2, NKX2-2 and IDBG-59106 and ENSG00000125820 and 4821, Nkx2-2 and IDBG-208906 and ENSMUSG00000027434 and 18088, nuclei, sequence-specific DNA binding, this GO :0001159 and core promoter proximal region DNA binding and molecular function this GO :0003323 and type B pancreatic cell development and biological process this GO :0003326 and pancreatic A cell fate commitment and biological process this GO :0003327 and type B pancreatic cell fate commitment and biological process this GO :0003329 and pancreatic PP cell fate commitment and biological process this GO :0003677 and DNA binding and molecular function this GO :0003682 and chromatin binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0003713 and transcription coactivator activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005622 and intracellular and cellular component this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0006351 and transcription, this GO :0001159: core promoter proximal region DNA binding, this GO :0001159: core promoter proximal region DNA binding and also this GO :0003677: DNA binding and also this GO :0003682: chromatin binding and also this GO :0003700: sequence-specific DNA binding transcription factor activity and also this GO :0003713: transcription coactivator activity and also this GO :0005515: protein binding and also this GO :0008134: transcription factor binding and also this GO :0043565: sequence-specific DNA binding, this GO :0003677: DNA binding, this GO :0003682: chromatin binding, this GO :0003700: sequence-specific DNA binding transcription factor activity, this GO :0003713: transcription coactivator activity, this GO :0005515: protein binding, this GO :0008134: transcription factor binding, this GO :0043565: sequence-specific DNA binding, 2 and NKX2B, 48094 and IDBG-639097 and ENSBTAG00000010277 and 531695, NK2 homeobox 2 |
| Identity | 7835 |
| Gene | NKX2-2 |
| Long gene name | NK2 homeobox 2 |
| Synonyms gene | NKX2B |
| Synonyms gene name | NK-2 (Drosophila) homolog B NK2 transcription factor related, locus 2 (Drosophila) |
| Locus | 20p11, 22 |
| Discovery year | 1992-06-12 |
| GenBank acession | AF019415 |
| Entrez gene record | 4821 |
| Pubmed identfication | 9703340 1346742 |
| Classification | NKL subclass homeoboxes and pseudogenes |
| Havana BLAST/BLAT | OTTHUMG00000170524 |
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