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NLK (1146CT24.2.1) Monoclonal Antibody

NLK (1146CT24.2.1) Monoclonal Antibody size: 0.1ml 211

Supplier BIOSS PRIMARY UNCONJUGATED ANTIBODIES
Price 211
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name1) Monoclonal Antibody, 2, NLK (1146CT24
Also known as1) Monoclonal Antibody, 2, NLK (1146CT24
CategoryPrimary Antibodies
ConjugationUnconjugated
Target AntigenNLK
SpecificityThis is a highly specific antibody against NLK
Modification(s)None
Modification site(s)Unmodified antibody
ClonalityMonoclonal
Clone number1, 1146CT24, 2
SourcePurified His-tagged NLK protein was used to produced this monoclonal antibody
Gene ID number51701
Swiss ProtQ9UBE8
Tested ApplicationsWB
Recommended dilutionsWB(1:1000)
Cross reactivityHuman
Cross reactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Background of the target antigen Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1, Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, Binds to and phosphorylates NOTCH1, Binds to and phosphorylates TCF7L2/TCF4 and LEF1, MAP3K7/TAK1 and HIPK2, Negative regulator of the MYB family of transcription factors, Negative regulator of the Notch signaling pathway, Negative regulator of the canonical Wnt/beta-catenin signaling pathway, Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself, Phosphorylates ATF5 but also stabilizes ATF5 protein levels in a kinase-independent manner (PubMed:25512613), Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP, Positive effector of the non-canonical Wnt signaling pathway, RBPJ/RBPSUH and MAML1, The NLK-SETDB1 complex subsequently interacts with PPARG, The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs), Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes, Upon IL1B stimulus, acting downstream of WNT5A, as well as the ubiquitination and subsequent proteolysis of LEF1, cooperates with ATF5 to activate the transactivation activity of C/EBP subfamily members, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, which is in turn required for activation of NLK by MAP3K7/TAK1, Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination
Purification method This antibody is purified through a protein G column, followed by dialysis against PBS, 09% (W/V) sodium azide, Purified monoclonal antibody supplied in PBS with 0
Storage conditions 50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA
Synonym names LAK1, NLK, Nemo-like kinase, Protein LAK1, Serine/threonine-protein kinase NLK
PropertiesC, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°
About Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Monoclonals of this antigen are available in different clones
Gene target1) Monoclonal, 2, NLK (1146CT24
Short name1) Monoclonal Antibody, 2, NLK (1146CT24
Technique Monoclonals or monoclonal antibodies, antibodies against human proteins, antibodies for, Antibody
Alternative name1) monoclonal (Antibody to), 2, nemo-like kinase (1146CT24
Alternative techniqueantibodies
Alternative to gene target DNA-templated and biological process this GO :0006355 and regulation of transcription, DNA-templated and biological process this GO :0006468 and protein phosphorylation and biological process this GO :0007179 and transforming growth factor beta receptor signaling pathway and biological process this GO :0008134 and transcription factor binding and molecular function this GO :0016055 and Wnt signaling pathway and biological process this GO :0016772 and transferase activity, NLK and IDBG-36215 and ENSG00000087095 and 51701, NLK and IDBG-631542 and ENSBTAG00000014825 and 507204, Nlk and IDBG-203133 and ENSMUSG00000017376 and 18099, nuclei, this GO :0000287 and magnesium ion binding and molecular function this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004707 and MAP kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0006351 and transcription, this GO :0000287: magnesium ion binding, this GO :0000287: magnesium ion binding and also this GO :0004672: protein kinase activity and also this GO :0004674: protein serine/threonine kinase activity and also this GO :0004707: MAP kinase activity and also this GO :0004713: protein tyrosine kinase activity and also this GO :0005515: protein binding and also this GO :0005524: ATP binding and also this GO :0008134: transcription factor binding and also this GO :0016772: transferase activity, this GO :0004672: protein kinase activity, this GO :0004674: protein serine/threonine kinase activity, this GO :0004707: MAP kinase activity, this GO :0004713: protein tyrosine kinase activity, this GO :0005515: protein binding, this GO :0005524: ATP binding, this GO :0008134: transcription factor binding, this GO :0016772: transferase activity, this GO :0031625: ubiquitin protein ligase binding, this GO :0042169: SH2 domain binding, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0031625: ubiquitin protein ligase binding and also this GO :0042169: SH2 domain binding, transferring phosphorus-containing groups and molecular function this GO :0018107 and peptidyl-threonine phosphorylation and biological process this GO :0030178 and negative regulation of Wnt signaling pathway and biological process this GO :0031625 and ubiquitin protein ligase binding and molecular function this GO :0033136 and serine phosphorylation of STAT3 protein and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0042169 and SH2 domain binding and molecular function this GO :0046777 and protein autophosphorylation and biological process, nemo-like kinase
Identity 29858
Gene NLK
Long gene name nemo like kinase
Synonyms gene name nemo-like kinase
Locus 17q11, 2
Discovery year 2004-02-19
GenBank acession AF197898
Entrez gene record 51701
Pubmed identfication 9448268 10863097
RefSeq identity NM_016231
Havana BLAST/BLAT OTTHUMG00000132451

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