| Long name | 1) Monoclonal Antibody, 2, NPM1 (1042CT5 |
| Also known as | 1) Monoclonal Antibody, 2, NPM1 (1042CT5 |
| Category | Primary Antibodies |
| Conjugation | Unconjugated |
| Target Antigen | NPM1 |
| Specificity | This is a highly specific antibody against NPM1 |
| Modification(s) | None |
| Modification site(s) | Unmodified antibody |
| Clonality | Monoclonal |
| Clone number | 1, 1042CT5, 2 |
| Source | Purified His-tagged NPM1 protein was used to produced this monoclonal antibody |
| Gene ID number | 4869 |
| Swiss Prot | P06748 |
| Tested Applications | IHC-P, WB |
| Recommended dilutions | WB(1:1000), IHC-P(1:25) |
| Cross reactivity | Human |
| Cross reactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen | Acts as a chaperonin for the core histones H3, Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486), Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids, Binds ribosome presumably to drive ribosome nuclear export, H2B and H4, In concert with BRCA2, May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules, Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation, Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication, Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA, and regulation of tumor suppressors p53/TP53 and ARF, cell proliferation, centrosome duplication, histone assembly, protein chaperoning, regulates centrosome duplication, Involved in diverse cellular processes such as ribosome biogenesis |
| Purification method | This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS, 09% (W/V) sodium azide, Purified monoclonal antibody supplied in PBS with 0 |
| Storage conditions | 50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names | NPM, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Nucleophosmin, Numatrin, B23 |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| About | Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Monoclonals of this antigen are available in different clones |
| Gene target | 1) Monoclonal, 2, NPM1 (1042CT5 |
| Short name | 1) Monoclonal Antibody, 2, NPM1 (1042CT5 |
| Technique | Monoclonals or monoclonal antibodies, antibodies against human proteins, antibodies for, Antibody |
| Alternative name | numatrin) (1042CT5, 1) monoclonal (Antibody to), 2, nucleophosmin (nucleolar phosphoprotein B23 |
| Alternative technique | antibodies |
| Alternative to gene target | B23 and NPM, NPM1 and IDBG-57453 and ENSG00000181163 and 4869, nuclei, numatrin), this GO :0003676 and nucleic acid binding and molecular function this GO :0003713 and transcription coactivator activity and molecular function this GO :0003723 and RNA binding and molecular function this GO :0004860 and protein kinase inhibitor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005730 and nucleolus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005813 and centrosome and cellular component this GO :0005829 and cytosol and cellular component this GO :0006281 and DNA repair and biological process this GO :0006334 and nucleosome assembly and biological process this GO :0006886 and intracellular protein transport and biological process this GO :0006913 and nucleocytoplasmic transport and biological process this GO :0006950 and response to stress and biological process this GO :0007098 and centrosome cycle and biological process this GO :0007165 and signal transduction and biological process this GO :0007569 and cell aging and biological process this GO :0008104 and protein localization and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0010826 and negative regulation of centrosome duplication and biological process this GO :0016020 and membrane and cellular component this GO :0016032 and viral process and biological process this GO :0019901 and protein kinase binding and molecular function this GO :0030529 and ribonucleoprotein complex and cellular component this GO :0030957 and Tat protein binding and molecular function this GO :0031616 and spindle pole centrosome and cellular component this GO :0032071 and regulation of endodeoxyribonuclease activity and biological process this GO :0034080 and centromere-specific nucleosome assembly and biological process this GO :0042255 and ribosome assembly and biological process this GO :0042393 and histone binding and molecular function this GO :0042803 and protein homodimerization activity and molecular function this GO :0043023 and ribosomal large subunit binding and molecular function this GO :0043024 and ribosomal small subunit binding and molecular function this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0044387 and negative regulation of protein kinase activity by regulation of protein phosphorylation and biological process this GO :0044822 and poly(A) RNA binding and molecular function this GO :0045727 and positive regulation of translation and biological process this GO :0046599 and regulation of centriole replication and biological process this GO :0046982 and protein heterodimerization activity and molecular function this GO :0051059 and NF-kappaB binding and molecular function this GO :0051082 and unfolded protein binding and molecular function this GO :0051092 and positive regulation of NF-kappaB transcription factor activity and biological process this GO :0051259 and protein oli this GO merization and biological process this GO :0060699 and regulation of endoribonuclease activity and biological process this GO :0060735 and regulation of eIF2 alpha phosphorylation by dsRNA and biological process, this GO :0003676: nucleic acid binding, this GO :0003676: nucleic acid binding and also this GO :0003713: transcription coactivator activity and also this GO :0003723: RNA binding and also this GO :0004860: protein kinase inhibitor activity and also this GO :0005515: protein binding and also this GO :0019901: protein kinase binding and also this GO :0030957: Tat protein binding and also this GO :0042393: histone binding and also this GO :0042803: protein homodimerization activity and also this GO :0043023: ribosomal large subunit binding and also this GO :0043024: ribosomal small subunit binding and also this GO :0044822: poly(A) RNA binding and also this GO :0046982: protein heterodimerization activity and also this GO :0051059: NF-kappaB binding and also this GO :0051082: unfolded protein binding, this GO :0003713: transcription coactivator activity, this GO :0003723: RNA binding, this GO :0004860: protein kinase inhibitor activity, this GO :0005515: protein binding, this GO :0019901: protein kinase binding, this GO :0030957: Tat protein binding, this GO :0042393: histone binding, this GO :0042803: protein homodimerization activity, this GO :0043023: ribosomal large subunit binding, this GO :0043024: ribosomal small subunit binding, this GO :0044822: poly(A) RNA binding, this GO :0046982: protein heterodimerization activity, this GO :0051059: NF-kappaB binding, this GO :0051082: unfolded protein binding, unfolded protein binding, nucleophosmin (nucleolar phosphoprotein B23 |
| Identity | 7910 |
| Gene | NPM1 |
| Long gene name | nucleophosmin |
| Synonyms gene name | nucleophosmin (nucleolar phosphoprotein B23, numatrin) |
| Synonyms | B23 NPM |
| Synonyms name | member 1 , nucleolar phosphoprotein B23 numatrin nucleophosmin/nucleoplasmin family |
| Locus | 5q35, 1 |
| Discovery year | 1993-08-24 |
| GenBank acession | M26697 |
| Entrez gene record | 4869 |
| Pubmed identfication | 8471164 8122112 |
| RefSeq identity | NM_002520 |
| Havana BLAST/BLAT | OTTHUMG00000130465 |
| Locus Specific Databases | LRG_458 |
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