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Oct4 Antibody

Oct4 Antibody size: 0.4 ml 406

Supplier NJS POLY
Price 406
Size 0.4 ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
CategoryAntibody
Concentration pH 7, with 0, 09% sodium azide, 4, In 1X PBS
FormPurified
ConjugationUnconjugated
ClonePolyclonal antibody
Recognised antigen38261
Host animalRabbit (Oryctolagus cuniculus)
ClonalityPolyclonal (rabbit origin)
Species reactivity Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens)
Tested applications ELISA, IF, WB
Recommended dilutionsImmunofluorescence: 1:50-1:100, Western blot: 1:1000
NotesTitration of the Oct4 antibody may be required due to differences in protocols and secondary/substrate sensitivity
Intented useThis Oct4 antibodyis to be used only for research purposes and not for diagnostics
Uniprot #Q01860
PurityPurified
Description Oct3 is present in mouse oocytes before and after fertilization, Reverse transcriptase PCR showed low level of expression in both OCT3A and OCT3B mRNA in all adult human tissues examined, Two forms of POU5F1/OCT4/OCT3 mRNA are expressed in adult tissues as a result of alternative splicing--OCT3A and OCT3B, When fertilized oocytes were injected with antisense Oct3 oligonucleotides or double-stranded DNA containing the octamer motif, embryonic DNA synthesis was inhibited and the embryos were arrested at the one-cell stage, Transcription factors containing the POU homeodomain have been shown to be important regulators of tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development
ImmunogenA portion of amino acids 1-30 from the human protein was used as the immunogen for this Oct4 antibody
Storage Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the Oct4 antibody and store frozen at -20 deg
PropertiesC, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°
Gene targetOct4
Short nameOct4 Antibody
Technique antibodies against human proteins, antibodies for, Antibody
Alternative nameOct4 (Antibody to)
Alternative techniqueantibodies
Identity 9221
Gene POU5F1
Long gene name POU class 5 homeobox 1
Synonyms gene OTF3
Synonyms gene name POU domain class 5, transcription factor 1
Synonyms OCT3 Oct4 MGC22487
Locus 6p21, 33
Discovery year 1992-11-05
GenBank acession Z11898
Entrez gene record 5460
Pubmed identfication 1408763
RefSeq identity NM_002701
Classification POU class homeoboxes and pseudogenes
Havana BLAST/BLAT OTTHUMG00000031206

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