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p150 CAF1 Antibody, Biotin Conjugated

p150 CAF1 Antibody, Biotin Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name Biotin Conjugated, p150 CAF1 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target Antigenp150 CAF1
SpecificityThis is a highly specific antibody against p150 CAF1
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human p150 CAF1
Gene ID number10036
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, Assembles histone octamers onto replicating DNA in vitro, CAF-1 performs the first step of the nucleosome assembly process, CHAF1A binds to histones H3 and H4, It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci (By similarity), The CCR4-NOT complex functions as general transcription regulation complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), a complex thought to mediate chromatin assembly in DNA replication and DNA repair, bringing newly synthesized histones H3 and H4 to replicating DNA, histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer, which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene, Core component of the CAF-1 complex
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetp150 CAF1 Conjugated
Short name Conjugated, p150 CAF1 Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, p150 CAF1 (Antibody to)
Alternative techniqueantibodies
Identity 14101
Gene CNOT7
Long gene name CCR4-NOT transcription complex subunit 7
Synonyms gene CAF1
Synonyms gene name CCR4-NOT transcription complex, subunit 7
Synonyms name BTG1 binding factor 1
Locus 8p22
Discovery year 2000-12-01
GenBank acession L46722
Entrez gene record 29883
Pubmed identfication 10637334 1538749 17264152
RefSeq identity NM_013354
Classification CCR4-NOT transcription complex
Havana BLAST/BLAT OTTHUMG00000096971

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