Name : p47 Antibody, FITC Conjugated
Supplier : BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price :333
SKU : GEN4756628050
| Long name | FITC Conjugated, p47 Polyclonal Antibody |
| Category | Conjugated Primary Antibodies |
| Conjugation | Anti-FITC Antibody |
| Host Organism | Rabbit (Oryctolagus cuniculus) |
| Target Antigen | p47 |
| Specificity | This is a highly specific antibody against p47 |
| Modification | Unmodified |
| Modification site | None |
| Clonality | Polyclonal |
| Clone | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Source | KLH conjugated synthetic peptide derived from human Pleckstrin/p47 |
| Gene ID number | 5341 |
| Tested applications | IF(IHC-P) |
| Recommended dilutions | IF(IHC-P)(1:50-200) |
| Crossreactivity | Mouse, Rat, Human |
| Crossreactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background | Functioning as part of a ternary complex with VCP (a protein involved in the heterotypic fusion of transport vesicles with their target membranes) and Syntaxin 5, Human p47 shares 89% sequence identity with its mouse counterpart, Multiple isoforms of p47 exist due to alternative splicing events, UBX1, UBXD10 or UBXN2C, also known as NSFL1C, is a 370 amino acid protein that localizes to both the nucleus and the golgi apparatus (specifically to golgi stacks) and contains one SEP domain and one UBX domain, p47 interacts with and reduces the ATPase activity of VCP and is required for the fragmentation of golgi stacks during mitosis and for subsequent reassembly of golgi stacks after mitosis, p47 is subject to phosphorylation during mitosis, required for proper golgi stack formation and cisternal regrowth, suggesting a conserved role between species, therefore, which inhibits p47-golgi interaction and is, Platelet P47 |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission | 494nm/518nm |
| Synonyms | FLJ27168, OTTHUMP00000159962, PLEK, PLEK_HUMAN, Platelet 47 kDa protein, Platelet P47, Platelet and leukocyte C kinase substrate, Platelet p47 protein, Pleckstrin, p47 |
| Properties | Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target | p47 Conjugated |
| Short name | FITC Conjugated, p47 Antibody |
| Technique | Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label | FITC |
| Alternative name | fluorecein coupled, p47 (Antibody to) |
| Alternative technique | antibodies |