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PAK4/5(Ser99) Antibody, ALEXA FLUOR 488

PAK4/5(Ser99) Antibody, ALEXA FLUOR 488 size: 100ul 350

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES
Price 350
Size 100ul
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name ALEXA FLUOR 488 Conjugated, PAK4/5(Ser99) Polyclonal Antibody
Also known asAnti-PAK4/5 Ser99 PAb ALEXA FLUOR 488
CategoryConjugated Primary Antibodies
Conjugated with 488, ALEXA FLUOR®
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenPAK4/5 Ser99
SpecificityThis is a highly specific antibody against PAK4/5 Ser99
ModificationPhosphorylation
Modification SiteSer99
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen range80-120/591
Subcellular location Mitochondrion, Nucleus, Cytoplasm
SourceKLH conjugated synthetic phosphopeptide derived from human PAK4 around the phosphorylation site of Ser99
Gene ID Number10298, 57144
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Cross-reactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Background of the antigen Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues, All PAK family members are characterized by the presence of p21-binding domain, Current data strongly implicates PAK-4 in oncogenesis, Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions, PAK4 is frequently overexpressed in human tumor cell lines of various tissue origins, Phosphorylates CTNND1, Phosphorylates the proto-oncogene RAF1 and stimulates its kinase activity, Phosphospecific directed against serine 474 detect activated PAK4 on the Golgi membrane when PAK4 is co-expressed with activated Cdc42, Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD, Serine (Ser-474) is the likely autophosphorylation site in the kinase domain of PAK4 in vivo, Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, and lipids, and transcription, apoptosis, cell migration, dynamics of the cytoskeleton, including the cell cycle, oncogenic transformation, p21-activated kinases are regulated by the small GTP-binding proteins Rac and Cdc42, probably to regulate cytoskeletal organization and cell morphology, proliferation or cell survival, which stimulate autophosphorylation and phosphorylation of exogenous substrates, p21-activated kinases (PAKs) belong to the family of serine/threonine kinases involved in the control of various cellular processes
PurificationPurified by Protein A
Storage conditions 50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA
Excitation emission499nm/519nm
Properties ALEXA FLUOR they should be stored frozen at - 24°, Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), Also Alexa Fluor 488 is pH stable, As a result of this photo stability, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, It is distinguished in the FL1 of a FACS-Calibur or FACScan, has a very high photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry, yet the PAK4/5(Ser99) Antibody, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
Gene targetPAK4/5(Ser99)
Short namePAK4/5(Ser99) Antibody
Technique antibodies against human proteins, antibodies for, Antibody
LabelALEXA FLUOR 488
Alternative name ALEXA FLUOR 488, PAK4/5(Ser99) (Antibody to)
Alternative techniqueantibodies
Identity 16059
Gene PAK4
Long gene name p21 (RAC1) activated kinase 4
Synonyms gene name p21(CDKN1A)-activated kinase 4 p21 protein (Cdc42/Rac)-activated kinase 4
Locus 19q13, 2
Discovery year 2001-07-12
GenBank acession AJ011855
Entrez gene record 10298
Pubmed identfication 9822598 10461188
Havana BLAST/BLAT OTTHUMG00000182800

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