PAK4/5(Ser99) Antibody, Biotin Conjugated

PAK4/5(Ser99) Antibody, Biotin Conjugated size: 0.1ml 350

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1

Price 350

Size 0.1ml

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Long name	Biotin Conjugated, PAK4/5(Ser99) Polyclonal Antibody
Category	Conjugated Primary Antibodies
Conjugation	Biotinylated
Host Organism	Rabbit (Oryctolagus cuniculus)
Target Antigen	PAK4/5 Ser99
Specificity	This phosphorylation site is homologous in the listed cross reactive species at the specified location, This is a highly specific antibody against PAK4/5 Ser99
Modification	Phosphorylation
Modification site	Ser99
Clonality	Polyclonal
Clone	Polyclonal antibody
Concentration	1ug per 1ul
Immunogen_range	80-120/591
Subcellular location	Mitochondrion, Nucleus, Cytoplasm
Source	KLH conjugated synthetic phosphopeptide derived from human PAK4 around the phosphorylation site of Ser99
Gene ID number	10298, 57144
Tested applications	IHC-P, WB
Recommended dilutions	IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity	Mouse, Rat, Human
Crossreactive species details	not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background	Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues, All PAK family members are characterized by the presence of p21-binding domain, Current data strongly implicates PAK-4 in oncogenesis, Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions, PAK4 is frequently overexpressed in human tumor cell lines of various tissue origins, Phosphorylates CTNND1, Phosphorylates the proto-oncogene RAF1 and stimulates its kinase activity, Phosphospecific directed against serine 474 detect activated PAK4 on the Golgi membrane when PAK4 is co-expressed with activated Cdc42, Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD, Serine (Ser-474) is the likely autophosphorylation site in the kinase domain of PAK4 in vivo, Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, and lipids, and transcription, apoptosis, cell migration, dynamics of the cytoskeleton, including the cell cycle, oncogenic transformation, p21-activated kinases are regulated by the small GTP-binding proteins Rac and Cdc42, probably to regulate cytoskeletal organization and cell morphology, proliferation or cell survival, which stimulate autophosphorylation and phosphorylation of exogenous substrates, p21-activated kinases (PAKs) belong to the family of serine/threonine kinases involved in the control of various cellular processes
Purification method	This antibody was purified via Protein A
Storage conditions	50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission	N/A
Properties	Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene target	PAK4/5(Ser99) Conjugated
Short name	Conjugated, PAK4/5(Ser99) Antibody
Technique	antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
Label	Biotin
Alternative name	biotinilated coupled, PAK4/5(Ser99) (Antibody to)
Alternative technique	antibodies
Identity	16059
Gene	PAK4
Long gene name	p21 (RAC1) activated kinase 4
Synonyms gene name	p21(CDKN1A)-activated kinase 4 p21 protein (Cdc42/Rac)-activated kinase 4
Locus	19q13, 2
Discovery year	2001-07-12
GenBank acession	AJ011855
Entrez gene record	10298
Pubmed identfication	9822598 10461188
Havana BLAST/BLAT	OTTHUMG00000182800

PAK4/5(Ser99) Antibody, Biotin Conjugated

PAK4/5(Ser99) Antibody, Biotin Conjugated size: 0.1ml 350

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