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PAK4/5(Ser99) Antibody, Cy5 Conjugated

PAK4/5(Ser99) Antibody, Cy5 Conjugated size: 0.1ml 368

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 368
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name Cy5 Conjugated, PAK4/5(Ser99) Polyclonal Antibody
CategoryConjugated Primary Antibodies
Conjugation5, Cy5
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenPAK4/5 Ser99
Specificity This phosphorylation site is homologous in the listed cross reactive species at the specified location, This is a highly specific antibody against PAK4/5 Ser99
ModificationPhosphorylation
Modification siteSer99
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range80-120/591
Subcellular location Mitochondrion, Nucleus, Cytoplasm
SourceKLH conjugated synthetic phosphopeptide derived from human PAK4 around the phosphorylation site of Ser99
Gene ID number10298, 57144
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues, All PAK family members are characterized by the presence of p21-binding domain, Current data strongly implicates PAK-4 in oncogenesis, Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions, PAK4 is frequently overexpressed in human tumor cell lines of various tissue origins, Phosphorylates CTNND1, Phosphorylates the proto-oncogene RAF1 and stimulates its kinase activity, Phosphospecific directed against serine 474 detect activated PAK4 on the Golgi membrane when PAK4 is co-expressed with activated Cdc42, Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD, Serine (Ser-474) is the likely autophosphorylation site in the kinase domain of PAK4 in vivo, Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, and lipids, and transcription, apoptosis, cell migration, dynamics of the cytoskeleton, including the cell cycle, oncogenic transformation, p21-activated kinases are regulated by the small GTP-binding proteins Rac and Cdc42, probably to regulate cytoskeletal organization and cell morphology, proliferation or cell survival, which stimulate autophosphorylation and phosphorylation of exogenous substrates, p21-activated kinases (PAKs) belong to the family of serine/threonine kinases involved in the control of various cellular processes
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission625, 650nm/670nm
Properties Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetPAK4/5(Ser99) Conjugated
Short name Conjugated, PAK4/5(Ser99) Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelCy5
Alternative name cyanine 5 coupled, PAK4/5(Ser99) (Antibody to)
Alternative techniqueantibodies
Identity 16059
Gene PAK4
Long gene name p21 (RAC1) activated kinase 4
Synonyms gene name p21(CDKN1A)-activated kinase 4 p21 protein (Cdc42/Rac)-activated kinase 4
Locus 19q13, 2
Discovery year 2001-07-12
GenBank acession AJ011855
Entrez gene record 10298
Pubmed identfication 9822598 10461188
Havana BLAST/BLAT OTTHUMG00000182800

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