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PAK4/5(Ser99) Antibody, FITC Conjugated

PAK4/5(Ser99) Antibody, FITC Conjugated size: 0.1ml 350

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 350
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, PAK4/5(Ser99) Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenPAK4/5 Ser99
Specificity This phosphorylation site is homologous in the listed cross reactive species at the specified location, This is a highly specific antibody against PAK4/5 Ser99
ModificationPhosphorylation
Modification siteSer99
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range80-120/591
Subcellular location Mitochondrion, Nucleus, Cytoplasm
SourceKLH conjugated synthetic phosphopeptide derived from human PAK4 around the phosphorylation site of Ser99
Gene ID number10298, 57144
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues, All PAK family members are characterized by the presence of p21-binding domain, Current data strongly implicates PAK-4 in oncogenesis, Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions, PAK4 is frequently overexpressed in human tumor cell lines of various tissue origins, Phosphorylates CTNND1, Phosphorylates the proto-oncogene RAF1 and stimulates its kinase activity, Phosphospecific directed against serine 474 detect activated PAK4 on the Golgi membrane when PAK4 is co-expressed with activated Cdc42, Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD, Serine (Ser-474) is the likely autophosphorylation site in the kinase domain of PAK4 in vivo, Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, and lipids, and transcription, apoptosis, cell migration, dynamics of the cytoskeleton, including the cell cycle, oncogenic transformation, p21-activated kinases are regulated by the small GTP-binding proteins Rac and Cdc42, probably to regulate cytoskeletal organization and cell morphology, proliferation or cell survival, which stimulate autophosphorylation and phosphorylation of exogenous substrates, p21-activated kinases (PAKs) belong to the family of serine/threonine kinases involved in the control of various cellular processes
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetPAK4/5(Ser99) Conjugated
Short name FITC Conjugated, PAK4/5(Ser99) Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, PAK4/5(Ser99) (Antibody to)
Alternative techniqueantibodies
Identity 16059
Gene PAK4
Long gene name p21 (RAC1) activated kinase 4
Synonyms gene name p21(CDKN1A)-activated kinase 4 p21 protein (Cdc42/Rac)-activated kinase 4
Locus 19q13, 2
Discovery year 2001-07-12
GenBank acession AJ011855
Entrez gene record 10298
Pubmed identfication 9822598 10461188
Havana BLAST/BLAT OTTHUMG00000182800

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