Name : PARD6G Antibody, FITC Conjugated
Supplier : BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price :333
SKU : GEN2195653822
| Long name | FITC Conjugated, PARD6G Polyclonal Antibody |
| Category | Conjugated Primary Antibodies |
| Conjugation | Anti-FITC Antibody |
| Host Organism | Rabbit (Oryctolagus cuniculus) |
| Target Antigen | PARD6G |
| Specificity | This is a highly specific antibody against PARD6G |
| Modification | Unmodified |
| Modification site | None |
| Clonality | Polyclonal |
| Clone | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Source | KLH conjugated synthetic peptide derived from human PARD6G |
| Tested applications | IF(IHC-P) |
| Recommended dilutions | IF(IHC-P)(1:50-200) |
| Crossreactivity | Mouse, Rat, Human |
| Crossreactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background | PARD6G contains one OPR domain, PARD6G is found at highest levels in adult and fetal kidney, PARD6G participates in the linking of GTP-bound Rho small GTPases to atypical protein kinase C (PKC) proteins, The PDZ and pseudo-CRIB domains are required for interaction with Rho small GTPases, This assembly is involved in formation of normal tight junctions at epithelial cell-cell contacts, Though widely expressed, Through its complex formation with PARD3G, When atypical PKC and PARD6G are expressed with a constitutively active RAC, one PDZ (DHR) domain and one pseudo-CRIB domain, the proteins colocalize to the membrane ruffles, which are structures that occur at the leading edge of polarized cells during movement, PARD6G (partitioning defective 6 homolog gamma) is a 376 amino acid adaptor protein that is involved in cell polarization and asymmetrical cell division processes |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission | 494nm/518nm |
| Synonyms | PAR-6G PAR6gamma |
| Properties | Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target | PARD6G Conjugated |
| Short name | FITC Conjugated, PARD6G Antibody |
| Technique | Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label | FITC |
| Alternative name | fluorecein coupled, PARD6G (Antibody to) |
| Alternative technique | antibodies |
| Identity | 16076 |
| Gene | PARD6G |
| Long gene name | par-6 family cell polarity regulator gamma |
| Synonyms gene name | C, elegans) , par-6 (partitioning defective 6, elegans) homolog gamma par-6 partitioning defective 6 homolog gamma (C |
| Locus | 18q23 |
| Discovery year | 2001-07-31 |
| Entrez gene record | 84552 |
| Pubmed identfication | 11260256 |
| RefSeq identity | NM_032510 |
| Classification | PDZ domain containing |
| Havana BLAST/BLAT | OTTHUMG00000132922 |