Filters

PCNA (1157CT1.1.3) Monoclonal Antibody

PCNA (1157CT1.1.3) Monoclonal Antibody size: 0.1ml 211

Supplier BIOSS PRIMARY UNCONJUGATED ANTIBODIES
Price 211
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name1, 3) Monoclonal Antibody, PCNA (1157CT1
Also known as1, 3) Monoclonal Antibody, PCNA (1157CT1
CategoryPrimary Antibodies
ConjugationUnconjugated
Target AntigenPCNA
SpecificityThis is a highly specific antibody against PCNA
Modification(s)None
Modification site(s)Unmodified antibody
ClonalityMonoclonal
Clone number1, 1157CT1, 3
SourceThis PCNA antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 236-261 amino acids from the C-terminal region of human PCNA
Gene ID number5111
Swiss ProtP12004
Tested Applications IHC-P, WB
Recommended dilutions WB(1:2000), IHC-P(1:25)
Cross reactivity Mouse, Rat, Human
Cross reactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Background of the target antigen APEX2 activities, Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, Has to be loaded onto DNA in order to be able to stimulate APEX2, Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902), but not apurinic-apyrimidinic (AP) endonuclease, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion, Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand
Purification method This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS, 09% (W/V) sodium azide, Purified monoclonal antibody supplied in PBS with 0
Storage conditions 50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA
Synonym names Cyclin, PCNA, Proliferating cell nuclear antigen, ATLD2
PropertiesC, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°
About Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Monoclonals of this antigen are available in different clones
Gene target1, 3) Monoclonal, PCNA (1157CT1
Short name1, 3) Monoclonal Antibody, PCNA (1157CT1
Technique Monoclonals or monoclonal antibodies, antibodies against human proteins, antibodies for, Antibody
Alternative name1, 3) monoclonal (Antibody to), PCNA (1157CT1
Alternative techniqueantibodies
Identity 8729
Gene PCNA
Long gene name proliferating cell nuclear antigen
Locus 20p12, 3
Discovery year 1990-01-15
GenBank acession J04718
Entrez gene record 5111
Pubmed identfication 2565339
Havana BLAST/BLAT OTTHUMG00000031798

Similar products

Subscribe to our Newsletter