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PCNA-Proliferation Marker Antibody, Biotin Conjugated

PCNA-Proliferation Marker Antibody, Biotin Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name Biotin Conjugated, PCNA-Proliferation Marker Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenPCNA-proliferation Marker
SpecificityThis is a highly specific antibody against PCNA-proliferation Marker
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range209-241/261
Subcellular location Helical, Nucleus
SourceKLH conjugated synthetic peptide derived from human PCNA
Gene ID number5111
Swiss ProtP12004
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background APEX2 activities, Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, Has to be loaded onto DNA in order to be able to stimulate APEX2, Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways, but not apurinic-apyrimidinic (AP) endonuclease, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion, Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetPCNA-Proliferation Conjugated
Short name Conjugated, PCNA-Proliferation Marker Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, PCNA-expantion detection labelled (Antibody to)
Alternative techniqueantibodies
Identity 8729
Gene PCNA
Long gene name proliferating cell nuclear antigen
Locus 20p12, 3
Discovery year 1990-01-15
GenBank acession J04718
Entrez gene record 5111
Pubmed identfication 2565339
Havana BLAST/BLAT OTTHUMG00000031798

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