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PDHA1(Ser293) Antibody, FITC Conjugated

PDHA1(Ser293) Antibody, FITC Conjugated size: 0.1ml 350

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 350
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, PDHA1(Ser293) Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenPDHA1 Ser293
Specificity Ser296 in Mouse, This antibody will detect PDHA2, This phosphorylation site is homologous across the listed species, and the predicted site of Ser296 in Rat due to sequence homology, the testis specific form, when phosphorylated at Ser293 in Human, This is a highly specific antibody against PDHA1 Ser293
ModificationPhosphorylation
Modification siteSer293
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range270-320/390
Subcellular locationMitochondrion
SourceKLH conjugated synthetic phosphopeptide derived from human PDHA1 around the phosphorylation site of Ser293
Gene ID number5160
Swiss ProtP08559
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background and thereby links the glycolytic pathway to the tricarboxylic cycle, The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2)
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetPDHA1(Ser293) Conjugated
Short name FITC Conjugated, PDHA1(Ser293) Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, PDHA1(Ser293) (Antibody to)
Alternative techniqueantibodies
Identity 8806
Gene PDHA1
Long gene name pyruvate dehydrogenase (lipoamide) alpha 1
Synonyms gene PDHA
Synonyms name mitochondrial , pyruvate dehydrogenase E1 component subunit alpha, somatic form
Locus Xp22, 12
Discovery year 1989-06-30
Entrez gene record 5160
Havana BLAST/BLAT OTTHUMG00000021224
Locus Specific Databases Mental Retardation database

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