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PDK1(Ser241) Antibody, FITC Conjugated

PDK1(Ser241) Antibody, FITC Conjugated size: 0.1ml 350

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 350
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, PDK1(Ser241) Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenPDK1 Ser241
Specificity This phosphorylation site is homologous to Ser244 in Mouse and Rat, This is a highly specific antibody against PDK1 Ser241
ModificationPhosphorylation
Modification siteSer241
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range220-260/556
Subcellular location Membrane bound, Cytoplasm
SourceKLH conjugated synthetic phosphopeptide derived from human PDK1 around the phosphorylation site of Ser241
Gene ID number5170
Swiss ProtO15530
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Activates PPARG transcriptional activity and promotes adipocyte differentiation, Activates the NF-kappa-B pathway via phosphorylation of IKKB, Controls proliferation, Essential for the motility of vascular endothelial cells (ECs) and is involved in the regulation of their chemotaxis, Its targets include: protein kinase B (PKB/AKT1, Negatively regulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor, PKB/AKT2, PKB/AKT3), Participates in the regulation of Ca(2+) entry and Ca(2+)-activated K(+) channels of mast cells, Plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, Plays a critical role in cardiac homeostasis by serving as a dual effector for cell survival and beta-adrenergic response, Plays an important role during thymocyte development by regulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cell growth and proliferative responses, RPS6KA2 and RPS6KA3), SGK2 and SGK3), SMAD3, SMAD4 and SMAD7, The tyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II, and growth of developing pancreatic cells, as well as glucose and amino acid uptake and storage, cyclic AMP-dependent protein kinase (PRKACA), p21-activated kinase-1 (PAK1), p70 ribosomal protein S6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, phosphorylating SMAD2, phosphorylating and activating a subgroup of the AGC family of protein kinases, preventing the nuclear translocation of SMAD3 and SMAD4 and the translocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta, protein kinase C (PRKCD and PRKCZ), protein kinase PKN (PKN1 and PKN2), serum and glucocorticoid-inducible kinase (SGK1, survival, thus propagating the signal to downstream targets controlling cell proliferation and survival, Serine/threonine kinase which acts as a master kinase
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetPDK1(Ser241) Conjugated
Short name FITC Conjugated, PDK1(Ser241) Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, PDK1(Ser241) (Antibody to)
Alternative techniqueantibodies
Identity 8809
Gene PDK1
Long gene name pyruvate dehydrogenase kinase 1
Synonyms gene name isoenzyme 1 pyruvate dehydrogenase kinase, isozyme 1 , pyruvate dehydrogenase kinase
Locus 2q31, 1
Discovery year 1996-08-14
GenBank acession L42450
Entrez gene record 5163
Pubmed identfication 7499431
RefSeq identity NM_002610
Havana BLAST/BLAT OTTHUMG00000132285

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