Filters

PDPK1(Ser410) Antibody, FITC Conjugated

PDPK1(Ser410) Antibody, FITC Conjugated size: 0.1ml 350

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 350
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, PDPK1(Ser410) Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenPDPK1 Ser410
SpecificityThis is a highly specific antibody against PDPK1 Ser410
ModificationPhosphorylation
Modification siteSer410
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic phosphopeptide derived from human PDPK1 around the phosphorylation site of Ser410
Gene ID number5170
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), Because of the role that PDK plays in insulin-induced glycogen synthesis and PKC activation it is a potentially important target for metabolic drug research, PDK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop, PDK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3, There are three named isoforms, eventually leading to the dephosphorylation and activation of glycogen synthase and the stimulation of glycogen synthesis, 4, 5-trisphosphate, PDK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms PDK1
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetPDPK1(Ser410) Conjugated
Short name FITC Conjugated, PDPK1(Ser410) Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, PDPK1(Ser410) (Antibody to)
Alternative techniqueantibodies
Identity 8816
Gene PDPK1
Long gene name 3-phosphoinositide dependent protein kinase 1
Synonyms name PkB kinase
Locus 16p13, 3
Discovery year 1998-03-23
GenBank acession AF017995
Entrez gene record 5170
Pubmed identfication 9094314 9445477
Havana BLAST/BLAT OTTHUMG00000128874

Similar products

Subscribe to our Newsletter