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PEA3 Antibody, Cy3 Conjugated

PEA3 Antibody, Cy3 Conjugated size: 0.1ml 350

Price 350
Size 0.1ml
Long name Cy3 Conjugated, PEA3 Polyclonal Antibody
CategoryConjugated Primary Antibodies
Conjugationcy3 conjugation kit
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenPEA3
SpecificityThis is a highly specific antibody against PEA3
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human PEA3
Gene ID number2118
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Drosophila E74, Each of these proteins recognizes similar motifs in DNA that share a centrally located 5'-GGAA-3' element, Ets-2 and Fli-1, For instance, It appears that all of the Ets proteins recognize the same central core sequence but that each protein interacts with unique sequences that flank this core, PEA3 binds the motif 5'-AGGAAG-3' (the PEA-3 motif), PEA3 is expressed at readily detectable levels in cells of epithelial and fibroblastic origin but is not expressed in hematopoietic cells, These include mouse PU, This is in contrast to other members of the Ets gene family, although PU, but does not bind to the sequence 5'-AGGAAC-3', chicken and human Ets-2 and rat GABP-a, each of which is expressed primarily in cells of hematopoietic origin, mouse and human Ets-1, recognized by PU, such as Ets-1, 1, 1, 1 binds equally well to both sequences, Several members of the Ets gene family are known to encode sequence-specific DNA binding proteins
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission512, 550nm/570, 615nm
Synonyms E1A-F E1AF PEA3
Properties 1 of the PEA3 Antibody, Conjugated can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC, Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser, This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetPEA3 Conjugated
Short name Conjugated, PEA3 Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelCy3
Alternative name cyanine 3 coupled, PEA3 (Antibody to)
Alternative techniqueantibodies
Identity 3493
Gene ETV4
Long gene name ETS variant 4
Synonyms gene name E1AF) , ets variant gene 4 (E1A enhancer-binding protein
Synonyms name E1A enhancer binding protein
Locus 17q21, 31
Discovery year 1995-03-27
GenBank acession U18018
Entrez gene record 2118
Pubmed identfication 8530053 1547944
RefSeq identity NM_001986
Classification ETS transcription factor family
Havana BLAST/BLAT OTTHUMG00000180884

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