Name : Phospho-E2F1 Antibody (pS332)
Supplier : NJS POLY
Price :406
SKU : GEN1253020255
| Category | Antibody |
| Concentration | pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form | Antigen affinity purified |
| Conjugation | Unconjugated |
| Clone | Polyclonal antibody |
| Recognised antigen | Phospho-E2F1 (pS332) |
| Host animal | Rabbit (Oryctolagus cuniculus) |
| Clonality | Polyclonal (rabbit origin) |
| Species reactivity | Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications | ELISA, DB |
| Recommended dilutions | Dot blot: 1:500 |
| Notes | Titration of the phospho-E2F1 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use | This phospho-E2F1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot # | Q01094 |
| Purity | Antigen affinity |
| Description | It binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner, It can mediate both cell proliferation and p53-dependent/independent apoptosis, E2F1 is a member of the E2F family of transcription factors |
| Immunogen | This phospho-E2F1 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS332 of human E2F1 |
| Storage | Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the phospho-E2F1 antibody and store frozen at -20 deg |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target | Phospho-E2F1 (pS332) |
| Short name | Phospho-E2F1 Antibody (pS332) |
| Technique | antibodies against human proteins, antibodies for, Antibody |
| Alternative name | phosphorilated-E2F transcription factor 1 (Antibody to) (pS332) |
| Alternative technique | antibodies |
| Alternative to gene target | BT, DNA-templated and biological process this GO :0006355 and regulation of transcription, DNA-templated and biological process this GO :0006915 and apoptotic process and biological process this GO :0007219 and Notch signaling pathway and biological process this GO :0007283 and spermatogenesis and biological process this GO :0008134 and transcription factor binding and molecular function this GO :0008283 and cell proliferation and biological process this GO :0008630 and intrinsic apoptotic signaling pathway in response to DNA damage and biological process this GO :0010628 and positive regulation of gene expression and biological process this GO :0030900 and forebrain development and biological process this GO :0035189 and Rb-E2F complex and cellular component this GO :0043276 and anoikis and biological process this GO :0043565 and sequence-specific DNA binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0045892 and negative regulation of transcription, DNA-templated and biological process this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0048146 and positive regulation of fibroblast proliferation and biological process this GO :0048255 and mRNA stabilization and biological process this GO :0051726 and regulation of cell cycle and biological process this GO :0071398 and cellular response to fatty acid and biological process this GO :0071456 and cellular response to hypoxia and biological process this GO :0071930 and negative regulation of transcription involved in G1/S transition of mitotic cell cycle and biological process this GO :0072332 and intrinsic apoptotic signaling pathway by p53 class mediator and biological process this GO :0097193 and intrinsic apoptotic signaling pathway and biological process this GO :1900740 and positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway and biological process this GO :1990086 and lens fiber cell apoptotic process and biological process this GO :2000045 and regulation of G1/S transition of mitotic cell cycle and biological process, E2F-1 and RBAP1 and RBBP3 and RBP3, E2F1 and IDBG-67214 and ENSG00000101412 and 1869, E2f1 and IDBG-210416 and ENSMUSG00000027490 and 13555, nuclei, sequence-specific DNA binding, this GO :0000077 and DNA damage checkpoint and biological process this GO :0000080 and mitotic G1 phase and biological process this GO :0000082 and G1/S transition of mitotic cell cycle and biological process this GO :0000085 and mitotic G2 phase and biological process this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0000278 and mitotic cell cycle and biological process this GO :0001047 and core promoter binding and molecular function this GO :0003677 and DNA binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0003714 and transcription corepressor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005667 and transcription factor complex and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0006351 and transcription, this GO :0001047: core promoter binding, this GO :0001047: core promoter binding and also this GO :0003677: DNA binding and also this GO :0003700: sequence-specific DNA binding transcription factor activity and also this GO :0003714: transcription corepressor activity and also this GO :0005515: protein binding and also this GO :0008134: transcription factor binding and also this GO :0043565: sequence-specific DNA binding, this GO :0003677: DNA binding, this GO :0003700: sequence-specific DNA binding transcription factor activity, this GO :0003714: transcription corepressor activity, this GO :0005515: protein binding, this GO :0008134: transcription factor binding, this GO :0043565: sequence-specific DNA binding, 78028 and IDBG-641855 and ENSBTAG00000003971 and 535369, E2F transcription factor 1 |
| Identity | 3113 |
| Gene | E2F1 |
| Long gene name | E2F transcription factor 1 |
| Synonyms gene | RBBP3 |
| Synonyms | RBP3 |
| Locus | 20q11, 22 |
| Discovery year | 1993-06-29 |
| Entrez gene record | 1869 |
| Pubmed identfication | 8964493 |
| Classification | E2F transcription factors |
| Havana BLAST/BLAT | OTTHUMG00000032265 |