Name : PI3KCA Antibody, FITC Conjugated
Supplier : BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price :333
SKU : GEN5921649278
| Long name | FITC Conjugated, PI3KCA Polyclonal Antibody |
| Category | Conjugated Primary Antibodies |
| Conjugation | Anti-FITC Antibody |
| Host Organism | Rabbit (Oryctolagus cuniculus) |
| Target Antigen | PI3KCA |
| Specificity | This is a highly specific antibody against PI3KCA |
| Modification | Unmodified |
| Modification site | None |
| Clonality | Polyclonal |
| Clone | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Immunogen_range | 1010-1050/1068 |
| Subcellular location | Membrane bound, Cytoplasm |
| Source | KLH conjugated synthetic peptide derived from human PI3KCA |
| Gene ID number | 5290 |
| Swiss Prot | P42336 |
| Tested applications | IF(IHC-P), FCM |
| Recommended dilutions | IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Crossreactivity | Mouse, Rat, Human |
| Crossreactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background | EIF4EBP1 and HRAS, Essential in endothelial cell migration during vascular development through VEGFA signaling, Has also serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), IGF1, Involved in signaling via insulin-receptor substrate (IRS) proteins, Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway, Participates in cellular signaling in response to various growth factors, Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway, PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4, Regulates invadopodia formation in breast cancer cells through the PDPK1-AKT1 pathway, Required for lymphatic vasculature development, VEGFA and PDGF, activating signaling cascades involved in cell growth, but not by PDGF, including AKT1 and PDPK1, insulin, motility and morphology, possibly by binding to RAS and by activation by EGF and FGF2, possibly by regulating RhoA activity, proliferation, survival, 4, 5)P2 (Phosphatidylinositol 4, 5-bisphosphate) to generate phosphatidylinositol 3, 5-trisphosphate (PIP3), Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol) |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission | 494nm/518nm |
| Synonyms | CLOVE, CWS5, MCAP, MCMTC, PI3-kinase subunit alpha, PI3K, PI3K-alpha, PI3Kalpha, PIK3CA, Phosphatidylinositol 4, Phosphatidylinositol 4, Phosphoinositide-3-kinase catalytic alpha polypeptide, PtdIns-3-kinase subunit alpha, PtdIns-3-kinase subunit p11-alpha, Serine/threonine protein kinase PIK3CA, p11-alpha, p11alpha, 5-bisphosphate 3-kinase 11 kDa catalytic subunit alpha, 5-bisphosphate 3-kinase catalytic subunit alpha isoform, MCM |
| Properties | Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target | PI3KCA Conjugated |
| Short name | FITC Conjugated, PI3KCA Antibody |
| Technique | Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label | FITC |
| Alternative name | fluorecein coupled, PI3KCA (Antibody to) |
| Alternative technique | antibodies |