Name : PIRT Antibody, FITC Conjugated
Supplier : BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price :333
SKU : GEN4269355817
| Long name | FITC Conjugated, PIRT Polyclonal Antibody |
| Category | Conjugated Primary Antibodies |
| Conjugation | Anti-FITC Antibody |
| Host Organism | Rabbit (Oryctolagus cuniculus) |
| Target Antigen | PIRT |
| Specificity | This is a highly specific antibody against PIRT |
| Modification | Unmodified |
| Modification site | None |
| Clonality | Polyclonal |
| Clone | Polyclonal antibody |
| Concentration | 1ug per 1ul |
| Source | KLH conjugated synthetic peptide derived from human PIRT |
| Tested applications | IF(IHC-P) |
| Recommended dilutions | IF(IHC-P)(1:50-200) |
| Crossreactivity | Mouse, Rat, Human |
| Crossreactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background | Although PIRT is expressed in peripheral nervous system, Correspondingly, Highly conserved among vertebrates, PIRT consists of two transmembrane domains and one putative C-terminal phosphoinositide-binding domain, PIRT is a required component of the VR1 complex, PIRT knockout results in impaired responses to noxious heat and capsaicin exposure, The gene that encodes PIRT maps to human chromosome 17p13, a sensor of both noxious heat and capsaicin, and lowest levels in sympathetic and enteric neurons, it is not expressed in spinal cord, which positively regulates VR1, while VR1 remains unaltered, with highest levels in dorsal root ganglion and trigeminal neurons, 1, PIRT is a 137 amino acid multi-pass membrane protein |
| Purification method | This antibody was purified via Protein A |
| Storage conditions | 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission | 494nm/518nm |
| Properties | Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target | PIRT Conjugated |
| Short name | FITC Conjugated, PIRT Antibody |
| Technique | Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label | FITC |
| Alternative name | fluorecein coupled, PIRT (Antibody to) |
| Alternative technique | antibodies |
| Identity | 37239 |
| Gene | PIRT |
| Long gene name | phosphoinositide interacting regulator of transient receptor potential channels |
| Synonyms gene name | phosphoinositide-interacting regulator of transient receptor potential channels |
| Synonyms name | phosphoinositide-interacting regulator of TRPV1 |
| Locus | 17p12 |
| Discovery year | 2010-06-04 |
| GenBank acession | AK021549 |
| Entrez gene record | 644139 |
| Pubmed identfication | 18455988 |
| RefSeq identity | NM_001101387 |
| Havana BLAST/BLAT | OTTHUMG00000178234 |