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PKR (Thr446) Antibody

PKR (Thr446) Antibody size: 0.1ml 281

Supplier BIOSS PRIMARY UNCONJUGATED ANTIBODIES
Price 281
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long namePKR (Thr446) Primary Polyclonal Antibody
Also known asPKR (Thr446) PAb
CategoryPrimary Antibodies
ConjugationUnconjugated
Target AntigenPKR Thr446
SpecificityThis is a highly specific antibody against PKR Thr446
Modification(s)Phosphorylation
Modification site(s)Thr446
ClonalityPolyclonal antibody
Clone numberPolyclonal antibody
Concentration1ug per 1ul
Immunogen range420-470/551
Subcellular location Nucleus, Cytoplasm
SourceKLH conjugated synthetic phosphopeptide derived from human PKR around the phosphorylation site of Thr446
Gene ID number5610
Swiss ProtP19525
Tested Applications IF(IHC-P), IHC-P, WB
Recommended dilutions IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000)
Cross reactivityHuman
Cross reactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Background of the target antigen ATF3) involved in the expression of genes encoding proinflammatory cytokines and IFNs, Activates the NF-kappa-B pathway via interaction with IKBKB and TRAF family of proteins and activates the p38 MAP kinase pathway via interaction with MAP2K6, Also phosphorylates other substrates including p53/TP53, Can act as both a positive and negative regulator of the insulin signaling pathway (ISP), DHX9, Either as an adapter protein and/or via its kinase activity, Exerts its antiviral activity on a wide range of DNA and RNA viruses including hepatitis C virus (HCV), ILF3, IRF1, IRS1 and the HHV-1 viral protein US11, In addition to serine/threonine-protein kinase activity, Inhibits viral replication via phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (EIF2S1), NF-kappa-B and insulin signaling pathways) and transcription factors (JUN, Negatively regulates ISP by inducing the inhibitory phosphorylation of insulin receptor substrate 1 (IRS1) at 'Ser-312' and positively regulates ISP via phosphorylation of PPP2R5A which activates FOXO1, PPP2R5A, STAT1, STAT3, also has tyrosine-protein kinase activity and phosphorylates CDK1 at 'Tyr-4' upon DNA damage, apoptosis, can regulate various signaling pathways (p38 MAP kinase, cell proliferation and differentiation, facilitating its ubiquitination and proteosomal degradation, hepatitis B virus (HBV), measles virus (MV) and herpes simplex virus 1 (HHV-1), this phosphorylation impairs the recycling of EIF2S1 between successive rounds of initiation leading to inhibition of translation which eventually results in shutdown of cellular and viral protein synthesis, which in turn up-regulates the expression of insulin receptor substrate 2 (IRS2), IFN-induced dsRNA-dependent serine/threonine-protein kinase which plays a key role in the innate immune response to viral infection and is also involved in the regulation of signal transduction
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA
Synonym names EIF2AK1, EIF2AK2, Eukaryotic translation initiation factor 2-alpha kinase 2, Interferon-induced, Interferon-inducible RNA-dependent protein kinase, P1/eIF-2A protein kinase, PPP1R83, PRKR, Protein kinase R, Protein kinase RNA-activated, Tyrosine-protein kinase EIF2AK2, double-stranded RNA-activated protein kinase, eIF-2A protein kinase 2, p68 kinase, PKR
PropertiesC, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°
Gene targetPKR (Thr446)
Short namePKR (Thr446) Antibody
Technique antibodies against human proteins, antibodies for, Antibody
Alternative namePKR (Thr446) (Antibody to)
Alternative techniqueantibodies
Identity 9437
Gene EIF2AK2
Long gene name eukaryotic translation initiation factor 2 alpha kinase 2
Synonyms gene PRKR
Synonyms gene name interferon-inducible double stranded RNA dependent , protein kinase
Synonyms PKR EIF2AK1 PPP1R83
Synonyms name protein phosphatase 1, regulatory subunit 83
Locus 2p22, 2
Discovery year 1992-12-04
GenBank acession BC057805
Entrez gene record 5610
Pubmed identfication 1351683
RefSeq identity NM_002759
Classification Protein phosphatase 1 regulatory subunits
Havana BLAST/BLAT OTTHUMG00000100962

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