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PMFBP1 Antibody, FITC Conjugated

PMFBP1 Antibody, FITC Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name FITC Conjugated, PMFBP1 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenPMFBP1
SpecificityThis is a highly specific antibody against PMFBP1
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human PMFBP1
Gene ID number83449
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background Due to evidence that PMFBP1 may play a role in sperm tail morphology, Localized to the nucleus, PMF-1 contains a coiled-coil domain which interacts with the leucine-zipper domain of Nrf2, PMF-1 is also a component of the MIS12 complex, PMF-1 is expressed at highest levels in skeletal muscle and heart, PMFBP1 (Polyamine-modulated factor 1-binding protein 1) is a 1022 amino acid protein that binds PMF-1 and may be involved in general organization of the cytoskeleton, There are three isoforms of PMFBP1 that are produced as a result of alternative splicing events, This interaction regulates the transcription of SSAT, a regulatory enzyme for polyamine catabolism, it may therefore affect fertility, which is required for kinetochore formation and chromosomal alignment and segregation, with moderate expression in liver and kidney, PMF-1 is a 205 amino acid protein involved in kinetochore formation
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetPMFBP1 Conjugated
Short name FITC Conjugated, PMFBP1 Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, PMFBP1 (Antibody to)
Alternative techniqueantibodies
Identity 17728
Gene PMFBP1
Long gene name polyamine modulated factor 1 binding protein 1
Locus 16q22, 2
Discovery year 2002-05-21
GenBank acession AF239683
Entrez gene record 83449
Pubmed identfication 11468771
RefSeq identity NM_031293
Havana BLAST/BLAT OTTHUMG00000167827

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