| Long name | 2) Monoclonal Antibody, 4, PRKAA2 (206CT5 |
| Also known as | 2) Monoclonal Antibody, 4, PRKAA2 (206CT5 |
| Category | Primary Antibodies |
| Conjugation | Unconjugated |
| Target Antigen | PRKAA2 |
| Specificity | This is a highly specific antibody against PRKAA2 |
| Modification(s) | None |
| Modification site(s) | Unmodified antibody |
| Clonality | Monoclonal |
| Clone number | 2, 206CT5, 4 |
| Source | This PRKAA2 monoclonal antibody is generated from mouse immunized with PRKAA2 recombinant protein |
| Gene ID number | 5563 |
| Swiss Prot | P54646 |
| Tested Applications | IF, WB |
| Recommended dilutions | IF(1:10~50), WB(1:1000) |
| Cross reactivity | Human |
| Cross reactive species details | not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen | AMPK is a heterotrimer consisting of an alpha catalytic subunit, AMPK is activated, AMPK is an important energy-sensing enzyme that monitors cellular energy status, In response to cellular metabolic stresses, Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia, and non-catalytic beta and gamma subunits, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol, The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK) |
| Purification method | This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS, 09% (W/V) sodium azide, Purified monoclonal antibody supplied in PBS with 0 |
| Storage conditions | 50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names | 5'-AMP-activated protein kinase catalytic subunit alpha-2, ACACA kinase, AMPK subunit alpha-2, AMPK2, AMPKa2, Acetyl-CoA carboxylase kinase, HMGCR kinase, Hydroxymethylglutaryl-CoA reductase kinase, PRKAA, PRKAA2, AMPK |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| About | Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Monoclonals of this antigen are available in different clones |
| Gene target | 2) Monoclonal, 4, PRKAA2 (206CT5 |
| Short name | 2) Monoclonal Antibody, 4, PRKAA2 (206CT5 |
| Technique | Monoclonals or monoclonal antibodies, antibodies against human proteins, antibodies for, Antibody |
| Alternative name | AMP-activated, a 2 catalytic subunit (206CT5, 2) monoclonal (Antibody to), 4, protein phosphorylation catalyst |
| Alternative technique | antibodies |
| Alternative to gene target | AMP-activated, AMPK and AMPK2 and AMPKa2 and PRKAA, DNA-templated and biological process this GO :0006355 and regulation of transcription, DNA-templated and biological process this GO :0006468 and protein phosphorylation and biological process this GO :0006633 and fatty acid biosynthetic process and biological process this GO :0006695 and cholesterol biosynthetic process and biological process this GO :0006853 and carnitine shuttle and biological process this GO :0006914 and autophagy and biological process this GO :0006950 and response to stress and biological process this GO :0007050 and cell cycle arrest and biological process this GO :0007165 and signal transduction and biological process this GO :0008286 and insulin receptor signaling pathway and biological process this GO :0008610 and lipid biosynthetic process and biological process this GO :0010508 and positive regulation of autophagy and biological process this GO :0016055 and Wnt signaling pathway and biological process this GO :0016772 and transferase activity, PRKAA2 and IDBG-638934 and ENSBTAG00000018747 and 538954, PRKAA2 and IDBG-99136 and ENSG00000162409 and 5563, Prkaa2 and IDBG-169837 and ENSMUSG00000028518 and 108079, alpha 2 catalytic subunit, nuclei, this GO :0003682 and chromatin binding and molecular function this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004679 and AMP-activated protein kinase activity and molecular function this GO :0004712 and protein serine/threonine/tyrosine kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0006112 and energy reserve metabolic process and biological process this GO :0006351 and transcription, this GO :0003682: chromatin binding, this GO :0003682: chromatin binding and also this GO :0004672: protein kinase activity and also this GO :0004674: protein serine/threonine kinase activity and also this GO :0004679: AMP-activated protein kinase activity and also this GO :0004712: protein serine/threonine/tyrosine kinase activity and also this GO :0004713: protein tyrosine kinase activity and also this GO :0005515: protein binding and also this GO :0005524: ATP binding and also this GO :0016772: transferase activity, this GO :0004672: protein kinase activity, this GO :0004674: protein serine/threonine kinase activity, this GO :0004679: AMP-activated protein kinase activity, this GO :0004712: protein serine/threonine/tyrosine kinase activity, this GO :0004713: protein tyrosine kinase activity, this GO :0005515: protein binding, this GO :0005524: ATP binding, this GO :0016772: transferase activity, this GO :0035174: histone serine kinase activity, this GO :0046872: metal ion binding, this GO :0047322: [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity, this GO :0050405: [acetyl-CoA carboxylase] kinase activity, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0035174: histone serine kinase activity and also this GO :0046872: metal ion binding and also this GO :0047322: [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity and also this GO :0050405: [acetyl-CoA carboxylase] kinase activity, transferring phosphorus-containing groups and molecular function this GO :0031669 and cellular response to nutrient levels and biological process this GO :0032007 and negative regulation of TOR signaling and biological process this GO :0035174 and histone serine kinase activity and molecular function this GO :0035404 and histone-serine phosphorylation and biological process this GO :0042149 and cellular response to glucose starvation and biological process this GO :0042304 and regulation of fatty acid biosynthetic process and biological process this GO :0042593 and glucose homeostasis and biological process this GO :0042752 and regulation of circadian rhythm and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0044255 and cellular lipid metabolic process and biological process this GO :0044281 and small molecule metabolic process and biological process this GO :0045821 and positive regulation of glycolytic process and biological process this GO :0046872 and metal ion binding and molecular function this GO :0047322 and [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity and molecular function this GO :0048511 and rhythmic process and biological process this GO :0050405 and [acetyl-CoA carboxylase] kinase activity and molecular function this GO :0055089 and fatty acid homeostasis and biological process this GO :0061024 and membrane organization and biological process this GO :2000505 and regulation of energy homeostasis and biological process, protein kinase |
| Identity | 9377 |
| Gene | PRKAA2 |
| Long gene name | protein kinase AMP-activated catalytic subunit alpha 2 |
| Synonyms gene | PRKAA |
| Synonyms gene name | AMP-activated, alpha 2 catalytic subunit , protein kinase |
| Synonyms | AMPK AMPKa2 |
| Locus | 1p32, 2 |
| Discovery year | 1995-12-12 |
| GenBank acession | BC069823 |
| Entrez gene record | 5563 |
| Pubmed identfication | 7959015 |
| RefSeq identity | NM_006252 |
| Havana BLAST/BLAT | OTTHUMG00000008282 |
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