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Protein A Antibody, Biotin Conjugated

Protein A Antibody, Biotin Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name Biotin Conjugated, Protein A Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenProtein A
SpecificityThis is a highly specific antibody against Protein A
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Subcellular locationExtracellular
SourceRecombinant protein A
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
CrossreactivityBacteria
Crossreactive species details aureus, S
Antigen background IgA and IgE as well as mouse IgG3 and IgG1, SPA binds proteins from many of mammalian species, SPA has been shown to bind with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b, SPA is also immobilized onto solid supports such as agarose beads for total IgG purifying or interest protein or protein complex identifying in immunoprecipitation studies, SPA is known to bind with Fc region of immunoglobulins preferentially through interaction with the VH3 variable region of the heavy chain, SPA is often produced in E, and helps inhibit phagocytic engulfment and acts as an immunological disguise via this type of interaction, biotin, coli and is practically coupled to other molecules such as enzymes, most notably IgGs, radioactive iodine for use in immunology and other biological research, thus the bacteria will disrupts opsonization and phagocytosis, whereas bind with moderate affinity to human IgM, Protein A is a 40-60 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus (SPA)
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms SPA, Staphylococcal protein A, gG-binding protein A, Immunoglobulin G binding protein A
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetProtein A Conjugated
Short name Conjugated, Protein A Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, Protein A (Antibody to)
Alternative techniqueantibodies

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