| Antigen detectable with this ELISA kit | Non Receptor Type 6 (PTPN6), Protein Tyrosine Phosphatase |
| Species reactivity | Homo sapiens (Human) |
| Range of detection | 0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity | 105ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application | ELISA |
| Sample types tested for this ELISA kit | Tissue homogenates and other biological fluids |
| Time for performing t hourse test | 4, 5 hoursours |
| Test principle | 10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Non Receptor Type 6 (PTPN6), Non Receptor Type 6 (PTPN6), Non Receptor Type 6 (PTPN6), Non Receptor Type 6 (PTPN6) in the samples is then determined by comparing the O, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Tyrosine Phosphatase, The concentration of Protein Tyrosine Phosphatase, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Tyrosine Phosphatase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Protein Tyrosine Phosphatase, D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling | Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory | Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties | E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test | ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target | Non Receptor 6 (PTPN6), Protein Tyrosine Phosphatase |
| Short name | Non Receptor 6 (PTPN6) ELISA kit, Protein Tyrosine Phosphatase |
| Technique | &hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host | antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name | Non Receptor classification 6 (protein tyrosine phosphatase, non-receptor classification 6) Enzyme-linked immunosorbent assay reagent, Protein Tyrosine Phosphatase |
| Alternative technique | elisas |
| Alternative to gene target | HCP and HCPH and HPTP1C and PTP-1C and SH-PTP1 and SHP-1 and SHP-1L and SHP1, PTPN6 and IDBG-15719 and ENSG00000111679 and 5777, PTPN6 and IDBG-640141 and ENSBTAG00000020294 and 512312, Ptpn6 and IDBG-187635 and ENSMUSG00000004266 and 15170, SH2 domain binding, non-receptor type 6, nuclei, this GO :0001784 and phosphotyrosine binding and molecular function this GO :0002244 and hematopoietic progenitor cell differentiation and biological process this GO :0002924 and negative regulation of humoral immune response mediated by circulating immunoglobulin and biological process this GO :0004725 and protein tyrosine phosphatase activity and molecular function this GO :0005001 and transmembrane receptor protein tyrosine phosphatase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005730 and nucleolus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005911 and cell-cell junction and cellular component this GO :0006470 and protein dephosphorylation and biological process this GO :0006915 and apoptotic process and biological process this GO :0007186 and G-protein coupled receptor signaling pathway and biological process this GO :0007596 and blood coagulation and biological process this GO :0008283 and cell proliferation and biological process this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0014068 and positive regulation of phosphatidylinositol 3-kinase signaling and biological process this GO :0016020 and membrane and cellular component this GO :0016311 and dephosphorylation and biological process this GO :0016791 and phosphatase activity and molecular function this GO :0017124 and SH3 domain binding and molecular function this GO :0018108 and peptidyl-tyrosine phosphorylation and biological process this GO :0019221 and cytokine-mediated signaling pathway and biological process this GO :0019901 and protein kinase binding and molecular function this GO :0030154 and cell differentiation and biological process this GO :0030220 and platelet formation and biological process this GO :0031295 and T cell costimulation and biological process this GO :0033277 and abortive mitotic cell cycle and biological process this GO :0033630 and positive regulation of cell adhesion mediated by integrin and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0035855 and megakaryocyte development and biological process this GO :0042105 and alpha-beta T cell receptor complex and cellular component this GO :0042130 and negative regulation of T cell proliferation and biological process this GO :0042169 and SH2 domain binding and molecular function this GO :0042267 and natural killer cell mediated cytotoxicity and biological process this GO :0043407 and negative regulation of MAP kinase activity and biological process this GO :0043409 and negative regulation of MAPK cascade and biological process this GO :0045087 and innate immune response and biological process this GO :0045577 and regulation of B cell differentiation and biological process this GO :0050732 and negative regulation of peptidyl-tyrosine phosphorylation and biological process this GO :0050853 and B cell receptor signaling pathway and biological process this GO :0050859 and negative regulation of B cell receptor signaling pathway and biological process this GO :0050860 and negative regulation of T cell receptor signaling pathway and biological process this GO :0050900 and leukocyte migration and biological process this GO :0051279 and regulation of release of sequestered calcium ion into cytosol and biological process this GO :0060333 and interferon-gamma-mediated signaling pathway and biological process this GO :0060334 and regulation of interferon-gamma-mediated signaling pathway and biological process this GO :0060337 and type I interferon signaling pathway and biological process this GO :0060338 and regulation of type I interferon-mediated signaling pathway and biological process this GO :0060397 and JAK-STAT cascade involved in growth hormone signaling pathway and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0070372 and regulation of ERK1 and ERK2 cascade and biological process this GO :0070527 and platelet aggregation and biological process this GO :2000045 and regulation of G1/S transition of mitotic cell cycle and biological process, this GO :0001784: phosphotyrosine binding, this GO :0001784: phosphotyrosine binding and also this GO :0004725: protein tyrosine phosphatase activity and also this GO :0005001: transmembrane receptor protein tyrosine phosphatase activity and also this GO :0005515: protein binding and also this GO :0016791: phosphatase activity and also this GO :0017124: SH3 domain binding and also this GO :0019901: protein kinase binding and also this GO :0042169: SH2 domain binding, this GO :0004725: protein tyrosine phosphatase activity, this GO :0005001: transmembrane receptor protein tyrosine phosphatase activity, this GO :0005515: protein binding, this GO :0016791: phosphatase activity, this GO :0017124: SH3 domain binding, this GO :0019901: protein kinase binding, this GO :0042169: SH2 domain binding, protein tyrosine phosphatase |
| Identity | 9658 |
| Gene | PTPN6 |
| Long gene name | non-receptor type 6 , protein tyrosine phosphatase |
| Synonyms | HCP HCPH PTP-1C SHP-1 SHP1 |
| Locus | 12p13, 31 |
| Discovery year | 1992-02-12 |
| Entrez gene record | 5777 |
| Pubmed identfication | 1639416 |
| RefSeq identity | NM_002831 |
| Classification | Protein tyrosine phosphatases, non-receptor type SH2 domain containing |
| Havana BLAST/BLAT | OTTHUMG00000168518 |